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Abnormal STEM Development in Maize nal/nal Mutant - A Confocal Microscopy Study

Published online by Cambridge University Press:  02 July 2020

Wayne Y. Cheng ,
Ping-chin Cheng  and
David B. Walden
Wayne Y. Cheng
Affiliation:
Williamsville East High School, Williamsville, NY14051, USA
Ping-chin Cheng
Affiliation:
Advanced Microscopy and Imaging laboratory, Department of Electrical Engineering, State University of New York, Buffalo, NY14260, USA
David B. Walden
Affiliation:
Department of Plant Sciences, University of Western Ontario, London, Ontario, CanadaN6A 5B7
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Extract

There are number of dwarf mutants in maize such as d8, dl and nal. The nana plant 1 (nal/nal) is characterized as short erect dwarf; the phenotype does not respond to treatment of giberellins. The recessive nal allele is located on the long arm of chromosome 3 (3L-113). In this study, we report the abnormal development of the stem vasculature and the lack of clearly defined nodes.

Maize (Zea mays L., var. Ohio 43 and nal mutant) plants were grown in 1999 summer nursery at the fieldstation of University of Western Ontario, London Ontario, Canada. Plants at approximately 2 months old were fixed in 1:3 acetic acid/ EtOH. Multiple changes of fixative were made for a period of a month to remove as much pigmentation as possible. The specimen was then washed thoroughly, stained in diluted Schiffs reagent (10%), washed in 0.1M metabisulphate solution (with 1 ml of 1 N HC1 per liter), dehydrated in EtOH and cleared in methyl salicylate.

Type
Light and Electron Microscopic Techniques for the Study of Plant Pathogenic Fungi and Their Interactions with Host Plants
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 692 - 693
Copyright
Copyright © Microscopy Society of America

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References

References:

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