Hostname: page-component-84b7d79bbc-fnpn6 Total loading time: 0 Render date: 2024-07-31T00:31:51.407Z Has data issue: false hasContentIssue false
  • English
  • Français

The Action of C-Type Natriuretic Peptide on Apoptosis and P53 Expression in Human Cardiomyocytes

Published online by Cambridge University Press:  02 July 2020

K. Seta ,
Y. Matsuda  and
C. Wei
K. Seta
Affiliation:
Cardiovascular Molecular Research, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
Y. Matsuda
Affiliation:
Cardiovascular Molecular Research, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Wei
Affiliation:
Cardiovascular Molecular Research, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
Get access

Extract

C-type natriuretic peptide (CNP) is endothelial cell origin and has potent vasodilatory and antimitogenic actions. We reported that CNP was markedly increased in human cardiac tissue with severe congestive heart failure. To date, the effects of CNP on cardiomyocyte growth and death remain unclear. Therefore, the present study was designed to investigate the actions of CNP on apoptosis and apoptosis-related gene p53 expression in human cardiomyocytes.

Human cardiac atrial tissue was obtained from open-heart surgery (n=6). The cardiac tissue was minced and incubated in the special tissue culture system in the absence or presence of CNP (10-7 M) for 24 hours. These studies were repeated with HS-142-1 (HS, 10-6 M), a natriuretic peptide biological receptor antagonist. To detect the DNA fragmentation, in situ terminal deoxymucleotidyl transferase dUTP nick end labeling (TUNEL) was performed. The p53 expression was determined by immunohistochemical staining (IHCS). An average of 1000 nuclei was analyzed for TUNEL and p53 staining studies.

Type
Apoptosis
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 622 - 623
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Wang, J.,etal. Science (1996) 273, 359–361.CrossRefGoogle Scholar
2.Wei, C., et al. Circulation (1993) 88, 1004–1009.CrossRefGoogle Scholar
3.Wu, C.F., et al. J Biol Chem (1997) 272, 14860–14868.CrossRefGoogle Scholar
4.Suenobu, N., et al. Arterioscler Thromb Vase Biol (1999) 19, 140–146.CrossRefGoogle Scholar
5. This research was supported in part by grants from the NIH (HL03174 & HL61299, C. Wei), AHAMD, NKF and University of Maryland School of Medicine.Google Scholar