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An Integrated Biological Imaging Facility: Capabilities of the Biological Microscopy and Image Reconstruction Resource

Published online by Cambridge University Press:  02 July 2020

J. Frank ,
C.A. Mannella  and
C. Rieder
J. Frank
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY12201-0509
C.A. Mannella
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY12201-0509
C. Rieder
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY12201-0509
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Extract

The Biological Microscopy and Image Reconstruction Resource (BMIRR) is operated by the Wadsworth Center as a national biotechnology resource, with funding through the NIH Center for Research Resources and from NSF. This biological imaging resource has evolved continuously over the past two decades. Early development focussed on correlative, same-cell light and electron microscopic techniques, combining the capabilities of video-enhanced light microscopy and high-voltage electron microscopy. A current area of development is electron microscopic tomography, whereby the full 3D capabilities of higher voltage (400-1200 KV) electron microscopy is brought to bear on biological problems. In particular, the recent development of techniques for merging projection data from two mutually perpendicular tilt series has permitted significantly improved resolution, reducing the missing wedge of information to a missing pyramid. Attention is now turning to optimization of conditions for applying tomography to frozen-hydrated specimens, using automated data collection on our cryo-IVEM. Combined with parallel advances in same-cell manipulation and viewing, the BMIRR provides biologists with a unique combination of imaging and computational tools for research into the 3D structure and dynamics that underly cellular processes (see figures and refs. 2-4).

Type
shared resources: access to critical instrumentation
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 271 - 272
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Penczek, P.et al., Ultramicroscopy 60(1995)393.10.1016/0304-3991(95)00078-XCrossRefGoogle Scholar
2.Mannella, C.A.et al., Trends Biochem. Sci. 22(1997)37.10.1016/S0968-0004(96)30050-9CrossRefGoogle Scholar
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7. The Biological Microscopy and Image Reconstruction Resource is funded by NIH grant RR01219 and NSF grant BIR-9219043.Google Scholar