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Correlative Instrumental Neutron Activation Analysis, LM, And TEM to Track in Vivo Distribution of Colloidal Gold Spheres in BALB/C Mice

Published online by Cambridge University Press:  02 July 2020

J.F. Hillyer  and
R.M. Albrecht
J.F. Hillyer
Affiliation:
Department of Animal Health and Biomedical Sciences, University of Wisconsin - Madison, Madison, WI53706
R.M. Albrecht
Affiliation:
Department of Animal Health and Biomedical Sciences, University of Wisconsin - Madison, Madison, WI53706
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Extract

Colloidal gold is routinely used conjugated to ligands or antibodies as a label for the detection of cell structures by transmission (TEM) and scanning (SEM) electron microscopy. Silver enhancement, a procedure that amplifies the size of gold spheres, is used for light microscopic (LM) observation of colloidal gold. These techniques are valuable in localization studies but it is difficult to quantitate the level of labeling in larger volumes of tissue solely by microscopic means.

Instrumental Neutron Activation Analysis (INAA) can be used to provide a quantitative index of gold accumulation at the organ and tissue levels. Given that gold is not naturally found in the body in any substantial amount and that colloidal gold and ligand conjugates can be prepared to yield uniform bead sizes, the amount of label can be determined at the organ and tissue levels in whole animal systems.

Type
Recent Advances in Labeling Techniques
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 998 - 999
Copyright
Copyright © Microscopy Society of America

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References

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5. This research was funded in part by the advanced opportunity fellowship program, University of Wisconsin - Madison. The aid of Paul Sims, Richard Cashwell, and Randall Massey is greatly appreciated.Google Scholar