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Effect of Mycotoxin-Containing Diets on Epigenetic Modifications of Mouse Oocytes by Fluorescence Microscopy Analysis

Published online by Cambridge University Press:  09 May 2014

Cheng-Cheng Zhu ,
Yan-Jun Hou ,
Jun Han ,
Hong-Lin Liu ,
Xiang-Shun Cui ,
Nam-Hyung Kim  and
Shao-Chen Sun
Cheng-Cheng Zhu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Yan-Jun Hou
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Jun Han
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Hong-Lin Liu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Xiang-Shun Cui
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
Nam-Hyung Kim
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
Shao-Chen Sun*
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
*
*Corresponding author. sunsc@njau.edu.cn
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Abstract

Mycotoxins, such as aflatoxin (AF), fumonisin B1, zearalenone (ZEA), and deoxynivalenol (DON), are commonly found in many food commodities. Mycotoxins have been shown to increase DNA methylation levels in a human intestinal cell line. We previously showed that the developmental competence of oocytes was affected in mice that had been fed a mycotoxin-containing diet. In this study, we explored possible mechanisms of low mouse oocyte developmental competence after mycotoxin treatment in an epigenetic modification perspective. Mycotoxin-contaminated maize (DON at 3,875 μg/kg, ZEA at 1,897 μg/kg, and AF at 806 μg/kg) was included in diets at three different doses (mass percentage: 0, 15, and 30%) and fed to mice for 4 weeks. The fluorescence intensity analysis showed that the general DNA methylation levels increased in oocytes from high dose mycotoxin-fed mice. Mouse oocyte histone methylation was also altered. H3K9me3 and H4K20me3 level increased in oocytes from mycotoxin-fed mice, whereas H3K27me3 and H4K20me2 level decreased in oocytes from mycotoxin-fed mice. Thus, our results indicate that naturally occurring mycotoxins have effects on epigenetic modifications in mouse oocytes, which may be one of the reasons for reduced oocyte developmental competence.

Keywords

mycotoxinepigeneticsDNA methylationhistone methylationoocyte
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 4 , August 2014 , pp. 1158 - 1166
Copyright
© Microscopy Society of America 2014 

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Footnotes

a

Equal contribution.

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