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Macrophage Targeted Photodynamic Regulation of Wound Healing

Published online by Cambridge University Press:  02 July 2020

Mariah Coleno ,
Vincent P. Wallace ,
Bruce J. Tromberg  and
Tayyaba Hasan
Mariah Coleno
Affiliation:
Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, Ca.92612
Vincent P. Wallace
Affiliation:
Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, Ca.92612
Bruce J. Tromberg
Affiliation:
Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, Ca.92612
Tayyaba Hasan
Affiliation:
Wellman Laboratories of Photomedicine, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts.
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Extract

Wound healing is coordinated by the diverse activities of cells of the monocyte/macrophage lineage.1 Macrophage depletion impairs wound healing, while overexpression of macrophage products is associated with chronic inflammatory states. If light-activatable pharmacologic agents could be selectively targeted to macrophages, clinical conditions resulting from overexpression such as postsurgical adhesions, hypertrophic scarring, and the formation of intimal hyperplasia could be suppressed. Similarly, adjusting light dose parameters to provide a stimulatory effect could selectively enhance wound repair processes.

To test these hypothesis, photoactivatable cytotoxins (photosensitizers, PS) coupled to specific receptor ligands have been evaluated for localization in cells found in cutaneous tissue using a non-invasive imaging method, two-photon microscopy.

The two-photon microscope (TPM) has been found to be a useful tool for imaging cells with submicron resolution thus allowing for the determination of photosensitizer localization within cells. Additional uses of the TPM will include imaging into epithelial tissue grown in culture and tracking cells in wounds.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1090 - 1091
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Riches, D.W.H.The Molecular and Cellular Biology of Wound Repair, Clark, R.A.F. and Henson, P.M. (eds.). New York: Plenum press, 1988.Google Scholar
2.Mertz, J., Xu, C and Webb, W.Optics Letters, vol. 20, no. 24: 2532–2534, 1995.CrossRefGoogle Scholar
3.Henderson, B.W., and Dougherty, T.J.. Photodynamic Therapy: Basic Principles and Clinical Applications. New York: Marcel Dekker, 1992.Google Scholar
4. This research was supported by The National Institutes of Health under Contract RR06961 and The Office of Naval Research under Contract N00014-94-0874.Google Scholar