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Microscopic Studies of Endospore and Parasporal Crystal In Bacillus Thuringiensis Kurstaki A3-4 From Fermentor

Published online by Cambridge University Press:  02 July 2020

K.C. Feng-Chen ,
X.S. Jan ,
B.L. Liu  and
Y.M. Tzeng
K.C. Feng-Chen
Affiliation:
Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC
X.S. Jan
Affiliation:
Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC
B.L. Liu
Affiliation:
Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC
Y.M. Tzeng
Affiliation:
Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC
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Extract

Using microbes for the biological control of pests is progressing very rapidly. Because of the high specificity and none of toxicity to the vertebrates, they become the environmental biopesticides. The most commonly used entomopathogen is Bacillus bacteria, such as B. thuringiensis.Mass culturing of B. thuringiensisin different types of fermentor is experimenting in order to reach the highest yield of endospore and the crystalline endotoxin, the main virulent components for the pesticidal activity.

Bacillus thuringiensisproduces endospore and the parasporal inclusions, Hannay (1953) observed in bacterium B. thuringiensis a mature endospore and a prism-shaped crystal (parasporal crystal).’ The endospore and the endotoxin crystal vary during growth depending upon the availability of nutrient. Glucose concentration in medium has enormous effects on the production of parasporal crystal, B. thuringiensiscan not grow, nor produce parasporal crystal in low concentration of glucose medium. The formation time of the endotoxin crystal also differs in different subspecies.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1128 - 1129
Copyright
Copyright © Microscopy Society of America

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References

1.Hannay, C. L., Nature 172(1953)1004.CrossRefGoogle Scholar
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