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Molecular Architecture of Ribonucleoprotein Vaults

Published online by Cambridge University Press:  02 July 2020

P. L. Stewart ,
L. H. Rome  and
L. B. Kong
P. L. Stewart
Affiliation:
Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology UCLA School of Medicine, Los Angeles, CA90095
L. H. Rome
Affiliation:
Department of Biological Chemistry UCLA School of Medicine, Los Angeles, CA90095
L. B. Kong
Affiliation:
Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology UCLA School of Medicine, Los Angeles, CA90095
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Extract

Vaults are ribonucleoprotein complexes highly conserved between species as diverse as Dictyostelium, rat, and human. Thousands of vault particles exist per cell in various tissues including liver, lung, kidney and spleen. These complexes have been found in the cytoplasm as well as associated with the nuclear membrane. Vaults have a large barrel-like middle section with a protruding cap on each end. These structures have an average mass of 12.9 ± 1 MDa, as measured by STEM, and are composed of 3 proteins, p210, pi92 and pi04, as well as an RNA strand of approximately 90 bases. The major vault protein, pl04, constitutes the majority of the particle mass, existing in 96 copies per structure. The major vault protein was recently recognized as the lung-resistance related protein (LRP), a known multidrug resistance gene that is over-expressed in certain cancer cells, including ovarian and myeloid leukemia cells.

A three-dimensional structure of the vault complex was generated using the QVIEW and IMAGIC-5 software packages (Fig. 1).

Type
Chambers and Channels: Functional Connections in Multiprotein Complexes Studied by Single Chambers and Channels
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 964 - 965
Copyright
Copyright © Microscopy Society of America

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References

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