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Multimodal Atomic Force Imaging of Open Hemichannelinduced Modulation of Cell Volume and Viscoelastic Properties

Published online by Cambridge University Press:  02 July 2020

Seung K. Rhee ,
Arjan P. Quist ,
Hai Lin ,
Nils Almqvist  and
Ratneshx Lai
Seung K. Rhee
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106 Department of Biochemistry, Yeungnam University, Kyongsan, Korea
Arjan P. Quist
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
Hai Lin
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
Nils Almqvist
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106 Department of Physics, Luleå University of Technology, S-971 87 Luleå, Sweden
Ratneshx Lai
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106 Corresponding author
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Extract

Hemichannels from two single cells can join upon contact between these cells to form gap junctions - an intercellular pathway for the direct exchange of ions and small metabolites. Using techniques of fluorescent dye-uptake assay, laser confocal fluorescence imaging and atomic force microscopy (AFM), we have examined the role of hemichannels, present in the non-junctional regions of single cell plasma membrane, in the modulation of cell volume.

Antibodies against a gap junctional protein connexin43, were immunolocalized to nonjunctional plasma membrane regions of single BICR-MlRk k (breast tumor epithelial) cells, KOM-1 (bovine aortic endothelial) cells, and GM04260 (AD-free human) fibroblast cells. In the absence of extracellular calcium, cytoplasmic uptake of Lucifer yellow (LY) but not of dextran-conjugated LY was observed in single cells. Dye uptake was prevented by gap junctional inhibitors, ẞ-glycyrrhetinic acid (ẞGCA) and oleamide.

Type
Biological Applications of Scanning Probe Microscopies
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 998 - 999
Copyright
Copyright © Microscopy Society of America

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