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Preliminary Identification of β-Carotene in the Vitreous Asteroid Bodies by Micro-Raman Spectroscopy and HPLC Analysis

Published online by Cambridge University Press:  01 March 2007

Shan-Yang Lin ,
Ko-Hua Chen ,
Wen-Ting Cheng ,
Chi-Tien Ho  and
Shun-Li Wang
Shan-Yang Lin
Affiliation:
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
Ko-Hua Chen
Affiliation:
Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan, Republic of China Medical Engineering Section, National Health Research Institute, Taipei, Taiwan, Republic of China
Wen-Ting Cheng
Affiliation:
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
Chi-Tien Ho
Affiliation:
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
Shun-Li Wang
Affiliation:
Department of Applied Chemistry, National ChiaYi University, ChiaYi, Taiwan, Republic of China
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Abstract

β-carotene was first identified from the vitreous asteroid bodies (ABs) excised from one patient with asteroid hyalosis (AH) by confocal Raman microspectroscopy and was also verified by high performance liquid chromatography (HPLC). Two patients had been diagnosed with AH and intervened by surgical vitrectomy due to blurred vision. The morphology and components of both AB specimens were observed by optical microscopy and determined by using confocal Raman microspectroscopy and HPLC analysis, respectively. Surprisingly, two unique peaks at 1528 and 1157 cm−1 were found in the Raman spectrum for the AB specimen of patient 1 alone, which were in close agreement with that of the Raman peaks at 1525 and 1158 cm−1 for β-carotene and/or lutein. However, HPLC analytical data clearly indicated that the retention time for the extracted sample from the AB specimen of patient 1 was observed at 13.685 min and just identical to that of β-carotene (13.759 min) rather than lutein (2.978 min). In addition, the lack of any peak in the HPLC profile for the AB specimen of patient 2 also confirmed the absence of Raman peaks at 1525 and 1158 cm−1. Thus this preliminary study strongly suggests that β-carotene as a unique component of ABs was specifically detected from the AB specimen of one AH patient by using confocal Raman microspectroscopy and HPLC analysis.

Keywords

asteroid hyalosisasteroid bodiesRamanHPLCβ-carotenelutein
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 13 , Issue 2 , April 2007 , pp. 128 - 132
Copyright
© 2007 Microscopy Society of America

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References

REFERENCES

Baena, J.R. & Lendl, B. (2004). Raman spectroscopy in chemical bioanalysis. Curr Opin Chem Biol 8, 534–539.Google Scholar
Bartlett, H. & Eperjesi, F. (2004). An ideal ocular nutritional supplement? Ophthalmic Physiol Opt 24, 339–349.Google Scholar
Bergren, R.L., Brown, G.C. & Duker, J.S. (1991). Prevalence and association of asteroid hyalosis with systemic diseases. Am J Ophthalmol 111, 289–293.Google Scholar
Bernstein, P.S., Yoshida, M.D., Katz, N.B., McClane, R.W. & Gellermann, W. (1998). Raman detection of macular carotenoid pigments in intact human retina. Invest Ophthalmol Vis Sci 39, 2003–2011.Google Scholar
Bernstein, P.S., Zhao, D.Y., Sharifzadeh, M., Ermakov, I.V. & Gellermann, W. (2004). Resonance Raman measurement of macular carotenoids in the living human eye. Arch Biochem Biophys 430, 163–169.Google Scholar
Billsten, H.H., Bhosale, P., Yemelyanov, A., Bernstein, P.S. & Polivka, T. (2003). Photophysical properties of xanthophylls in carotenoproteins from human retinas. Photochem Photobiol 78, 138–145.Google Scholar
Centeno, J.A., Mullick, F.G., Panos, R.G., Miller, F.W. & Valenzuela-Espinoza, A. (1999). Laser-Raman microprobe identification of inclusions in capsules associated with silicone gel breast implants. Mod Pathol 12, 714–721.Google Scholar
Chen, K.H., Cheng, W.T., Li, M.J., Yang, D.M. & Lin, S.Y. (2005). Calcification of senile cataractous lens determined by Fourier transform infrared (FTIR) and Raman microspectroscopies. J Microsc 219, 36–41.Google Scholar
Cheng, W.T., Liu, M.T., Liu, H.N. & Lin, S.Y. (2005). Micro-Raman spectroscopy used to identify and grade human skin pilomatrixoma. Microsc Res Technol 68, 75–79.Google Scholar
Fawzi, A.A., Vo, B., Kriwanek, R., Ramkumar, H.L., Cha, C., Carts, A., Heckenlively, J.R., Foos, R.Y. & Glasgow, B.J. (2005). Asteroid hyalosis in an autopsy population: The University of California at Los Angeles (UCLA) experience. Arch Ophthalmol 123, 486–490.Google Scholar
Feldman, G.L. (1967). Human ocular lipids: Their analysis and distribution. Surv Ophthalmol 12, 207–243.Google Scholar
Gellermann, W., Ermakov, I.V., Ermakova, M.R., McClane, R.W., Zhao, D.Y. & Bernstein, P.S. (2002). In vivo resonant Raman measurement of macular carotenoid pigments in the young and the aging human retina. J Opt Soc Am A Opt Image Sci Vis 19, 1172–1186.Google Scholar
Hanlon, E.B., Manoharan, R., Koo, T.W., Shafer, K.E., Motz, J.T., Fitzmaurice, M., Kramer, J.R., Itzkan, I., Dasari, R.R. & Feld, M.S. (2000). Prospects for in vivo Raman spectroscopy. Phys Med Biol 45, R1–R59.Google Scholar
Hogg, R. & Chakravarthy, U. (2004). AMD and micronutrient antioxidants. Curr Eye Res 29, 387–401.Google Scholar
Khachik, F., Carvalho, L., Bernstein, P.S., Muir, G.J., Zhao, D.Y. & Katz, N.B. (2002). Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Exp Biol Med 227, 845–851.Google Scholar
Komatsu, H., Kamura, Y., Ishi, K. & Kashima, Y. (2003). Fine structure and morphogenesis of asteroid hyalosis. Med Electron Microsc 36, 112–119.Google Scholar
Lakshminarayana, R., Raju, M., Krishnakantha, T.P. & Baskaran, V. (2005). Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography. J Agric Food Chem 53, 2838–2842.Google Scholar
Lin, S.Y., Chen, K.H., Li, M.J., Cheng, W.T. & Wang, S.L. (2004). Evidence of octacalcium phosphate and Type-B carbonated apatites deposited on the surface of explanted acrylic hydrogel intraocular lens. J Biomed Mater Res 70B, 203–208.Google Scholar
Miller, H., Miller, B., Rabinowitz, H., Zonis, S. & Nir, I. (1983). Asteroid bodies—An ultrastructural study. Invest Ophthalmol Vis Sci 24, 133–136.Google Scholar
Mitchell, P., Wang, M.Y. & Wang, J.J. (2003). Asteroid hyalosis in an older population: The Blue Mountains Eye Study. Ophthalmic Epidemiol 10, 331–335.Google Scholar
Morganti, P., Fabrizi, G. & Bruno, C. (2004). Protective effects of oral antioxidants on skin and eye function. Skinmed 3, 310–316.Google Scholar
Moss, S.E., Klein, R. & Klein, B.E. (2001). Asteroid hyalosis in a population: The Beaver Dam eye study. Am J Ophthalmol 132, 70–75.Google Scholar
Notingher, I. & Hench, L.L. (2006). Raman microspectroscopy: A noninvasive tool for studies of individual living cells in vitro. Expert Rev Med Devices 3, 215–234.Google Scholar
Petry, R., Schmitt, M. & Popp, J. (2003). Raman spectroscopy—A prospective tool in the life sciences. Chemphyschem 4, 14–30.Google Scholar
Ramanauskaite, R.B., Segers-Nolten, G.M.J., de Graw, K.J., Sijtsema, N.M., van der Maas, L., Greve, J., Otto, C. & Fidgor, C.G. (1997). Carotenoid levels in human lymphocytes, measured by Raman microspectroscopy. Pure Appl Chem 69, 2131–2134.Google Scholar
Stahl, W. & Sies, H. (2005). Bioactivity and protective effects of natural carotenoids. Biochim Biophys Acta 1740, 101–107.Google Scholar
Streeten, B.W. (1982). Vitreous asteroid bodies. Ultrastructural characteristics and composition. Arch Ophthalmol 100, 969–975.Google Scholar
Topilow, H.W., Kenyon, K.R., Takahashi, M., Freeman, H.M., Tolentino, F.I. & Hanninen, L.A. (1982). Asteroid hyalosis. Biomicroscopy, ultrastructure, and composition. Arch Ophthalmol 100, 964–968.Google Scholar
Weesie, R.J., Merlin, J.C., Lugtenburg, J., Britton, G., Jansen, F.J. & Cornard, J.P. (1999). Semiempirical and Raman spectroscopic studies of carotenoids. Biospectroscopy 5, 19–33.Google Scholar
Winkler, J. & Lunsdorf, H. (2001). Ultrastructure and composition of asteroid bodies. Invest Ophthalmol Vis Sci 42, 902–907.Google Scholar
Withnall, R., Chowdhry, B.Z., Silver, J., Edwards, H.G. & de Oliveira, L.F. (2003). Raman spectra of carotenoids in natural products. Spectrochim Acta A Mol Biomol Spectrosc 59, 2207–2212.Google Scholar
Yazar, Z., Hanioglu, S., Karakoc, G. & Gursel, E. (2001). Asteroid hyalosis. Eur J Ophthalmol 11, 57–61.Google Scholar
Zhao, D.Y., Wintch, S.W., Ermakov, I.V., Gellermann, W. & Bernstein, P.S. (2003). Resonance Raman measurement of macular carotenoids in retinal, choroidal, and macular dystrophies. Arch Ophthalmol 121, 967–972.Google Scholar