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Scanning Force Microscopy and Nanomangulation: Studies of Dna and Proteins Involved in Dna Repair

Published online by Cambridge University Press:  02 July 2020

Dorothy Erie ,
Glenn Ratcliff ,
Martin Guthold ,
Valerie Bullock ,
Michelle Pliske ,
Richard Superfine  and
Russell Taylor
Dorothy Erie
Affiliation:
Chemistry Department, University of North Carolina, Chapel Hill, NC27599
Glenn Ratcliff
Affiliation:
Chemistry Department, University of North Carolina, Chapel Hill, NC27599
Martin Guthold
Affiliation:
Chemistry Department, University of North Carolina, Chapel Hill, NC27599 Department of Physics and Astronomy, University of North Carolina, Chapel Hill, NC27599 Computer Science Department, University of North Carolina, Chapel Hill, NC27599
Valerie Bullock
Affiliation:
Chemistry Department, University of North Carolina, Chapel Hill, NC27599
Michelle Pliske
Affiliation:
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC27599
Richard Superfine
Affiliation:
Department of Physics and Astronomy, University of North Carolina, Chapel Hill, NC27599
Russell Taylor
Affiliation:
Computer Science Department, University of North Carolina, Chapel Hill, NC27599
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Extract

Repair of damaged or incorrectly matched DNA is essential to the survival of all organisms. Consequently cells have devised a plentitude of pathways for repair. We have been investigating the mechanisms of mismatch repair and base excision repair. Both of these repair processes involve a large number of proteins that interact with one another as well as with DNA. Our long-term goal is to assemble complexes that are fully functional for DNA repair and to image the process of DNA repair. In addition, we wish to i) determine the stoicheometry of binding of the protein complexes to each other and to DNA, ii) monitor conformational changes due to substrate binding, iii) measure physical properties of DNA and the complexes. To accomplish this end, we have endeavored to improve techniques for solution imaging as well as those for data analysis. In this presentation I will discuss data on the stoicheometry of binding in several protein complexes and data on the physical properties of DNA.

To measure the physical properties of DNA, we utilize a nanoManipulator, a modified Scanning Force Microscope with a novel, user-friendly interface that allows easy and controlled manipulation of nanometer-sized samples.

Type
Biological Applications of Scanning Probe Microscopies
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1004 - 1005
Copyright
Copyright © Microscopy Society of America

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References

1 Falvo, M., Superfine, R., Washburn, S., Finch, M., Taylor, R. M., Chi, V. L., Brooks, F. P. Jr., “The Nanomanipulator: A Teleoperator for Manipulating Materials at the Nanometer Scale”, Proceedings of the International Symposium on the Science and Technology ofAtomically Engineered Materials (Richmond, VA, Oct 30 -Nov 4, 1995). World Scientific Publishing. 1996. pp. 579–586.Google Scholar

2 Ratcliff, G., Superfine, R., and Erie, D.A. “Photothermal Modulation for Oscillating Mode Atomic Force Microscopy in Solution,” Applied Physics Letters, 72, 1911–1913.CrossRefGoogle Scholar