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Structural Analysis of Filaments Using the STEM

Published online by Cambridge University Press:  02 July 2020

M. N. Simon ,
B. Y. Lin  and
J. S. Wall
M. N. Simon
Affiliation:
Department of Biology, Brookhaven National Laboratory, Upton, NY11973
B. Y. Lin
Affiliation:
Department of Biology, Brookhaven National Laboratory, Upton, NY11973
J. S. Wall
Affiliation:
Department of Biology, Brookhaven National Laboratory, Upton, NY11973
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Extract

The Scanning Transmission Electron Microscope (STEM) facility at BNL is an NIH Biotechnology Research Resource and as such is available to users with suitable projects, free of charge. Currently, many of our users' projects involve studying the structures of a variety of filaments. Most of these are of biological origin, although a couple involve conducting polymers. The STEM has a long history of being used to study different types of filaments and resolving controversies about their structure.

The mainstay of the STEM is mass analysis on unstained, isolated, freeze-dried samples. On these, the STEM can collect in-focus digital data directly. In a scan (8 sec. in real time) of a sample, at each of 512x512 picture elements (pixels), the number of electrons scattered into two annular detectors is recorded. For each pixel, the number of scattered electrons is directly proportional to the mass thickness in that pixel.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 862 - 863
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Steven, A. C. et al., PNAS (USA) 79, 3101–3105 (1982).CrossRefGoogle Scholar
2.Cerritelli, M. E. et al., J. Mol. Biol. 260, 767–780 (1996).CrossRefGoogle Scholar
3.Citovsky, V. et al., J. Mol. Biol. 271, 718–727 (1997).CrossRefGoogle Scholar
4. The BNL STEM is a NIH Supported Resource Center, NIH P41-RR01777, with additional support by DOE.OBER.Google Scholar