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Structural and Biomechanical Effects of Photooxidative Collagen Cross-Linking with Photosensitizer Riboflavin and 370 nm UVA Light on Human Corneoscleral Tissues

Published online by Cambridge University Press:  06 June 2013

Samjin Choi ,
Jae-Ho Shin ,
Youjin Cheong ,
Kyung-Hyun Jin  and
Hun-Kuk Park
Samjin Choi
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Korea Department of Medical Engineering, Kyung Hee University, Seoul 130-701, Korea
Jae-Ho Shin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul 130-701, Korea
Youjin Cheong
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Korea
Kyung-Hyun Jin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul 130-701, Korea
Hun-Kuk Park*
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Korea Department of Medical Engineering, Kyung Hee University, Seoul 130-701, Korea
*
*Corresponding author. E-mail: sigmoidus@khu.ac.kr
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Abstract

This study quantitatively investigated the immediate effects of a photooxidative collagen cross-linking treatment with photosensitizer riboflavin (RF) and 370 nm UVA light in in vitro human corneoscleral collagen fibrils using histology, thickness, scanning electron microscopy, and atomic force microscopy analyses. Twenty 8 × 2 mm corneoscleral strips were dissected sagittally from donor tissue using a scalpel. Four parameters were investigated, including the density, thickness, adhesion force, and stiffness of corneoscleral tissues before and after the collagen cross-linking treatment. The RFUVA-catalyzed collagen cross-linking treatment led to an increase in the density of both corneal (8%) and scleral (23%) stromal collagens. However, there was no difference in corneoscleral thickness. Furthermore, RFUVA-catalyzed collagen cross-linking treatment led to an increased biomechanical response of corneosclera: 25 and 8% increases in corneoscleral stiffness, and 24 and 22% increases in corneoscleral adhesion force. The collagen cross-linking treatment through RF-sensitized photoreaction may cause structural and biomechanical changes in the collagen fibril network of the cornea and the sclera. This is due to narrowing of the interfibrillar spacing and the stromal edema.

Keywords

photooxidative collagen cross-linking treatmentriboflavin and ultraviolet A lightkeratoconusmyopiacorneoscleral collagensatomic force microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1334 - 1340
Copyright
Copyright © Microscopy Society of America 2013 

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References

Agrawal, V. (2009). Corneal collagen cross-linking with riboflavin and ultraviolet-A light for keratoconus: Results in Indian eyes. Ind J Ophthalmol 57, 111–114.Google Scholar
Al-Torbak, A.A., Al-Motowa, S., Al-Assiri, A., Al-Kharashi, S., Al-Shahwan, S., Al-Mezaine, H. & Teichmann, K. (2006). Deep anterior lamellar keratoplasty for keratoconus. Cornea 25, 408–412.Google Scholar
Ambekar, R., Toussaint, K.C. & Wagoner Johnson, A. (2011). The effect of keratoconus on the structural, mechanical, and optical properties of the cornea. J Mech Behav Biomed Mater 4, 223–236.Google Scholar
Bell, G.R. (1993). Biomechanical considerations of high myopia: Part I—Physiological characteristics. J Am Optom Assoc 64, 332–338.Google Scholar
Choi, S., Cheong, Y., Lee, H.J., Lee, S.J., Jin, K.H. & Park, H.K. (2011a). AFM study for morphological and material property of human sclera surface. J Nanosci Nanotechnol 11, 6382–6388.Google Scholar
Choi, S., Lee, S.J., Shin, J.H., Cheong, Y., Lee, H.J., Paek, J.H., Kim, J.S., Jin, K.H. & Park, H.K. (2011b). Ultrastructural investigation of intact orbital implant surfaces using atomic force microscopy. Scanning 33, 211–221.Google Scholar
Choi, S., Shin, J.H., Cheong, Y., Lee, H.J., Jin, K.H. & Park, H.K. (2011c). Nanostructural investigation of frontalis sling biomaterial surfaces. Scanning 33, 419–425.Google Scholar
Edwards, M.H., Li, R.W., Lam, C.S., Lew, J.K. & Yu, B.S. (2002). The Hong Kong progressive lens myopia control study: Study design and main findings. Invest Ophthalmol Vis Sci 43, 2852–2858.Google Scholar
Gefen, A., Shalom, R., Elad, D. & Mandel, Y. (2009). Biomechanical analysis of the keratoconic cornea. J Mech Behav Biomed Mater 2, 224–236.CrossRefGoogle ScholarPubMed
Gwiazda, J., Hyman, L., Hussein, M., Everett, D., Norton, T.T., Kurtz, D., Leske, M.C., Manny, R., Marsh-Tootle, W. & Scheiman, M. (2003). A randomized clinical trial of progressive addition lenses versus single vision lenses on the progression of myopia in children. Invest Ophthalmol Vis Sci 44, 1492–1500.CrossRefGoogle ScholarPubMed
Jung, G.B., Lee, H.J., Kim, J.H., Lim, J.I., Choi, S., Jin, K.H. & Park, H.K. (2011). Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera. J Biomed Opt 16, 125004. Google Scholar
Katz, J., Schein, O.D., Levy, B., Cruiscullo, T., Saw, S.M., Rajan, U., Chan, T.K., Yew Khoo, C. & Chew, S.J. (2003). A randomized trial of rigid gas permeable contact lenses to reduce progression of children's myopia. Am J Ophthalmol 136, 82–90.Google Scholar
Kymionis, G.D., Diakonis, V.F., Kalyvianaki, M., Portaliou, D., Siganos, C., Kozobolis, V.P. & Pallikaris, A.I. (2009). One-year follow-up of corneal confocal microscopy after corneal cross-linking in patients with post laser in situ keratosmileusis ectasia and keratoconus. Am J Ophthalmol 147, 774–778.Google Scholar
Mao, J.R. & Bristow, J. (2001). The Ehlers–Danlos syndrome: On beyond collagens. J Clin Invest 107, 1063–1069.CrossRefGoogle ScholarPubMed
McCall, A.S., Kraft, S., Edelhauser, H.F., Kidder, G.W., Lundquist, R.R., Bradshaw, H.E., Dedeic, Z., Dionne, M.J., Clement, E.M. & Conrad, G.W. (2010). Mechanisms of corneal tissue cross-linking in response to treatment with topical riboflavin and long-wavelength ultraviolet radiation (UVA). Invest Ophthalmol Vis Sci 51, 129–138.Google Scholar
Meek, K.M. & Fullwood, N.J. (2001). Corneal and scleral collagens—A microscopist's perspective. Micron 32, 261–272.CrossRefGoogle ScholarPubMed
Rada, J.A., Shelton, S. & Norton, T.T. (2006). The sclera and myopia. Exp Eye Res 82, 185–200.Google Scholar
Raiskup-Wolf, F., Hoyer, A., Spoerl, E. & Pillunat, L.E. (2008). Collagen crosslinking with riboflavin and ultraviolet-A light in keratoconus: Long-term results. J Cataract Refract Surg 34, 796–801.Google Scholar
Siatkowski, R.M., Cotter, S.A., Crockett, R.S., Miller, J.M., Novack, G.D., Zadnik, K. & U.S. Pirenzepine Study Group (2008). Two-year multicenter, randomized, double-masked, placebo-controlled, parallel safety and efficacy study of 2% pirenzepine ophthalmic gel in children with myopia. J AAPOS 12, 332–339.Google Scholar
Siatkowski, R.M., Cotter, S., Miller, J.M., Scher, C.A., Crockett, R.S., Novack, G.D. & US Pirenzepine Study Group (2004). Safety and efficacy of 2% pirenzepine ophthalmic gel in children with myopia: A 1-year, multicenter, double-masked, placebo-controlled parallel study. Arch Ophthalmol 122, 1667–1674.Google Scholar
Spoerl, E., Wollensak, G. & Seiler, T. (2004). Increased resistance of crosslinked cornea against enzymatic digestion. Curr Eye Res 29, 35–40.Google Scholar
Vinciguerra, P., Albe, E., Trazza, S., Rosetta, P., Vinciguerra, R., Seiler, T. & Epstein, D. (2009). Refractive, topographic, tomographic, and aberrometric analysis of keratoconic eyes undergoing corneal cross-linking. Ophthalmology 116, 369–378.CrossRefGoogle ScholarPubMed
Wittig-Silva, C., Whiting, M., Lamoureux, E., Lindsay, R.G., Sullivan, L.J. & Snibson, G.R. (2008). A randomized controlled trial of corneal collagen cross-linking in progressive keratoconus: Preliminary results. J Refract Surg 34, S720–S725.Google Scholar
Wollensak, G. & Iomdina, E. (2009). Long-term biomechanical properties of rabbit sclera after collagen crosslinking using riboflavin and ultraviolet A (UVA). Acta Ophthalmol 87, 193–198.CrossRefGoogle ScholarPubMed
Wollensak, G., Iomdina, E., Dittert, D.D., Salamatina, O. & Stoltenburg, G. (2005). Cross-linking of scleral collagen in the rabbit using riboflavin and UVA. Acta Ophthalmol Scand 83, 477–482.Google Scholar
Wollensak, G. & Redl, B. (2008). Gel electrophoretic analysis of corneal collagen after photodynamic cross-linking treatment. Cornea 27, 353–356.Google Scholar
Wollensak, G. & Spoerl, E. (2004). Collagen crosslinking of human and porcine sclera. J Cataract Refract Surg 30, 689–695.CrossRefGoogle ScholarPubMed