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Structural Diversity of Aspergillus (Section Nigri) Spores

Published online by Cambridge University Press:  10 June 2013

Marta Filipa Simões ,
Cledir Santos  and
Nelson Lima
Marta Filipa Simões
Affiliation:
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
Cledir Santos
Affiliation:
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
Nelson Lima*
Affiliation:
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
*
*Corresponding author. E-mail: nelson@ie.uminho.pt
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Abstract

The taxonomy of fungal species, similar to that of many other microorganisms, suffers frequent revisions due to the discovery of new species and to the development and gathering of characterization data and morphological information. Morpho-taxonomy helps in the identification of many species. This work presents the macro, micro-morphological, and spectral mass analyses for phenotypic characterization of 13 species of Aspergillus section Nigri, showing that the characterization of spores (conidia) by scanning electron microscopy can be used as a tool to discriminate key morphological characteristics and separate closely related fungi. These results were corroborated by colony plates, stereomicroscopy, light microscopy, and spectral mass data.

Keywords

Aspergillus section Nigrifungal taxonomymorpho-taxonomyspore characterization
Type
Portuguese Society for Microscopy
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1151 - 1158
Copyright
Copyright © Microscopy Society of America 2013 

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References

Abarca, M.L., Accensi, F., Cano, J. & Cabañes, F.J. (2004). Taxonomy and significance of black aspergilli. Antonie van Leeuwenhoek 86, 33–49.CrossRefGoogle ScholarPubMed
de Vries, R.P., Frisvad, J.C., van de Vondervoort, P.J., Burgers, K., Kuijpers, A.F., Samson, R.A. & Visser, J. (2005). Aspergillus vadensis, a new species of the group of black Aspergilli. Antonie van Leeuwenhoek 87, 195–203.CrossRefGoogle Scholar
Geiser, D.M., Klich, M.A., Frisvad, J.C., Peterson, S.W., Varga, J. & Samson, R.A. (2007). The current status of species recognition and identification in Aspergillus . Stud Mycol 59, 1–10.Google Scholar
Hubka, V. & Loralik, M. (2012). β-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: Primer specificity testing and taxonomic consequences. Persoonia 29, 1–10.CrossRefGoogle ScholarPubMed
Jurjević, Z., Peterson, S.W., Stea, G., Solfrizzo, M., Varga, J., Hubka, V. & Perrone, G. (2012). Two novel species of Aspergillus section Nigri from indoor air. IMA Fungus 3, 159–173.Google Scholar
Klich, M.A. (2002). Identification of Common Aspergillus Species. The Netherlands: Centraalbureau voor Schimmelautures.Google Scholar
Kozakiewicz, Z. (1989). Aspergillus Species on Stored Products. Mycological Papers 161. Wallingford, UK: CABI Publishing.Google Scholar
Paterson, R.R.M., Venâncio, A. & Lima, N. (2004). Solutions to Penicillium taxonomy crucial to mycotoxins research and health. Res Microbiol 155, 507–513.Google Scholar
Paterson, R.R.M., Venâncio, A. & Lima, N. (2006). A practical approach for identification based on mycotoxin characters of Penicillium . Rev Iberoam Micol 23, 155–159.CrossRefGoogle ScholarPubMed
Perrone, G., Varga, J., Susca, A., Frisvad, J., Stea, G., Kocsube, S., Toth, B., Kozakiewicz, Z. & Samson, R. (2008). Aspergillus uvarum sp. nov., an uniseriate black Aspergillus species isolated from grapes in Europe. Int J Syst Evol Microbiol 58, 1032–1039.Google Scholar
Rodrigues, P., Santos, C., Venâncio, A. & Lima, N. (2011). Species identification of Aspergillus section Flavi isolates from Portuguese almonds using phenotypic, including MALDI-TOF ICMS, and molecular approaches. J Appl Microbiol 111, 877–892.CrossRefGoogle ScholarPubMed
Rodrigues, P., Soares, C., Kozakiewicz, Z., Paterson, R.R.M., Lima, N. & Venâncio, A. (2007). Identification and characterization of Aspergillus flavus and aflatoxins. In Communicating Current Research and Educational Topics and Trends in Applied Microbiology, Méndez-Vilas, A. (Ed.), pp. 527–534. Badajoz, Spain: Formatex.Google Scholar
Samson, R.A., Houbraken, J.A.M.P., Kuijpers, A.F.A., Frank, M.J. & Frisvad, J.C. (2004). New ochratoxin A or sclerotium producing species in Aspergillus section Nigri . Stud Mycol 50, 45–61.Google Scholar
Samson, R.A., Noonim, P., Meijer, M., Houbraken, J., Frisvad, J.C. & Varga, J. (2007). Diagnostic tools to identify black Aspergilli. Stud Mycol 59, 129–145.Google Scholar
Santos, C., Paterson, R.R.M., Venâncio, A. & Lima, N. (2010). Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Appl Microbiol 108, 375–385.Google Scholar
Serra, R., Cabañes, F.J., Perrone, G., Castella, G., Venâncio, A., Mule, G. & Kozakiewickz, Z. (2006). Aspergillus ibericus: A new species of section Nigri isolated from grapes. Mycologia 98, 295–306.Google Scholar
Simões, M.F., Pereira, L., Santos, C. & Lima, N. (2013). Polyphasic identification and preservation of fungal diversity: Concepts and applications. In Management of Microbial Resources in the Environment, Malik, A., Grohmann, E. & Alves, M. (Eds.), pp. 91–118. Dordrecht: Springer.CrossRefGoogle Scholar
Varga, J., Frisvad, J.C., Kocsubé, S., Brankovics, B., Tóth, B., Szigeti, G. & Samson, R.A. (2011). New and revisited species in Aspergillus section Nigri . Stud Mycol 69, 1–17.Google Scholar
Varga, J., Kocsubé, S., Tóth, B., Frisvad, J.C., Perrone, G., Susca, A., Meijer, M. & Samson, R.A. (2007). Aspergillus brasiliensis sp. nov., a biseriate black Aspergillus species with world-wide distribution. Int J Syst Evol Microbiol 57, 1925–1932.Google Scholar