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Tridimensional Characteristics of Rat Testicles Submitted to Tumour Cells Implant (Walker 256)

Published online by Cambridge University Press:  02 July 2020

R.P. CHOPARD ,
M.G. LOURENCO  and
R.P. OCAÑA
R.P. CHOPARD
Affiliation:
Department of Anatomy of the Institute of Biomedical Sciences of Sao Paulo University, Santos
M.G. LOURENCO
Affiliation:
Department of Anatomy of the Institute of Biomedical Sciences of Sao Paulo University, Santos
R.P. OCAÑA
Affiliation:
Santa Cecilia University- , Santos
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Extract

The intention of this paper is to comparatively demonstrate the tridimensional architecture of the microvascularization and the pattern of the microvascularization of testicles submitted to tumour cells implant, considering that supporting mechanisms of cell vitality depend on the vascular array.

Testicles of 25 Wistar male rats were used. To proceed the implant of the tumour cells it was used a 50cc insulin syringe with short needle witch was introduced into the inferior apex of the organ. The animals were anaesthetised with 50% ethylic ether solution and 50% alcohol 96° by inhalation in proper chamber. The weight of the rats was verified every day to evaluate alterations due to tumour development and malignant evolution so it was possible to determine the exact day of sacrifice.

The perfusion was performed trough the abdominal part of the aorta using a needle connected to a silicon tube.

Type
Applications and Advances in Vascular Corrosion Casting in Microvascular Research
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 572 - 573
Copyright
Copyright © Microscopy Society of America

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References

References:

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