Hostname: page-component-5c6d5d7d68-thh2z Total loading time: 0 Render date: 2024-08-19T04:23:23.124Z Has data issue: false hasContentIssue false
  • English
  • Français

The Ultrastructure of Cadaverous Skin after Cryopreservation with Polyethylene Oxide-400

Published online by Cambridge University Press:  02 July 2020

R. Migunova ,
B. Sandomirski  and
A. Kaprelyants
R. Migunova
Affiliation:
Department of cryocytology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, 61015, Ukraine Email:kaprelyants@yahoo.com
B. Sandomirski
Affiliation:
Department of cryomedicine, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, 61015, Ukraine
A. Kaprelyants
Affiliation:
Department of cryocytology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, 61015, Ukraine Email:kaprelyants@yahoo.com
Get access

Extract

The cryopreservation is the method of selection of long-term skin preservation used for the closure of large wound defects and the only possibility of long preservation of graft skin material in the viable state. For the lowtemperature preservation of human skin was successfully used cryoprotectant Polyethylene oxide -400, which was confirmed by the clinical evaluation during autotransplantation /1/. At the same time many aspects of the influence of low temperatures on the ultrastructural skin components were unclear /2/. The investigation of subcellular elements during step of low-temperature preservation specifies mechanisms of influence on the tissue preservation factors and determine the condition of skin cellular elements /3,4,5/. The aim of this work is to study ultrastructural characteristics of human cadaverous skin during the stages of low-temperature preservation by PEO-400.

In this investigation epidermal skin grafts with the thickness of 0.4-0.6 mm were studied in 6 hours after the accidental death divided in 3 series:

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 592 - 593
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Sandomirski, B.P., Kaprelyants, A.S., Proc. VII Sci. Conf. on the Problem of “Burns”, Leningrad (1981)122–123.Google Scholar
2.Kaprelyants, A.S., Pushkar, N.S., Cell Ultrastructure at Low Temperatures, Naukova Dumka Publ, Ukraine( 1978) 180 (In Russian).Google Scholar
3.Kaprelyants, A.S., Proc. Problem Der Hematologic, Transfusion und Transplantation, Berlin (1976) 156–161.Google Scholar
4.Kaprelyants, A.S., Proc. 28th Annual Meeting “CRYO-91”, K.U. Leuven (1991) 532.Google Scholar
5.Yurchenko, T.N., Cooling Effect on Cell Structure.Naukova Dumka Publ,Ukraine (1992) 148.Google Scholar
6. Programmable Cooling Device. Pat. Ukraine No. 19188.Google Scholar
7. EM-Preparation Chamber. Pat. Ukraine No. 1194137.Google Scholar
8. Device for Preparation of Biological Systems. Pat. Germany No 262561.Google Scholar
9. Equipment for Preparation of Biological Systems. Pat. Ukraine No. 119070.Google Scholar
10.Kaprelyants, A.S., Introduction Into Electron-Microscopic Technique for BioMedical Investigations, Naukova Dumka Publ, Ukraine (1982) 199.Google Scholar