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Uranium Microdistribution in Renal Cortex of Rats after Chronic Exposure: A Study by Secondary Ion Mass Spectrometry Microscopy

Published online by Cambridge University Press:  05 January 2012

Christine Tessier ,
David Suhard ,
François Rebière ,
Maâmar Souidi ,
Isabelle Dublineau  and
Michelle Agarande
Christine Tessier*
Affiliation:
Institut de Radioprotection et de Sûreté Nucléaire (IRSN), DRPH, SDI, LRC, BP 17, F-92262 Fontenay aux Roses Cedex, France
David Suhard
Affiliation:
Institut de Radioprotection et de Sûreté Nucléaire (IRSN), DRPH, SDI, LRC, BP 17, F-92262 Fontenay aux Roses Cedex, France
François Rebière
Affiliation:
Institut de Radioprotection et de Sûreté Nucléaire (IRSN), DRPH, SDI, LRC, BP 17, F-92262 Fontenay aux Roses Cedex, France
Maâmar Souidi
Affiliation:
Institut de Radioprotection et de Sûreté Nucléaire (IRSN), DRPH, SRBE, LRTOX, BP 17, F-92262 Fontenay aux Roses Cedex, France
Isabelle Dublineau
Affiliation:
Institut de Radioprotection et de Sûreté Nucléaire (IRSN), DRPH, SRBE, LRTOX, BP 17, F-92262 Fontenay aux Roses Cedex, France
Michelle Agarande
Affiliation:
Institut de Radioprotection et de Sûreté Nucléaire (IRSN), DRPH, SDI, LRC, BP 17, F-92262 Fontenay aux Roses Cedex, France
*
Corresponding author. E-mail: christine.tessier@irsn.fr
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Abstract

For a few years, the biological effects on ecosystems and the public of the bioaccumulation of radionuclides in situations of chronic exposures have been studied. This work, in keeping with the ENVIRHOM French research program, presents the uranium microdistribution by secondary ion mass spectrometry (SIMS) technique in the renal cortex of rats following chronic exposure to this low level element in the drinking water (40 mg/L) as a function to exposure duration (6, 9, 12, and 18 months). The SIMS mass spectra and 238U+ ion images produced with a SIMS CAMECA 4F-E7 show the kinetic of uranium accumulation in the different structures of the kidney. For the rats contaminated up to 12 months, the radioelement is mainly fixed in the proximal tubules; then after 18 exposure months, uranium is detected in all the segments of the nephron. This work has also shown that ion microscopy is an analytical method to detect trace elements and give elemental cartography at the micrometer scale.

Keywords

SIMSuraniumchronic contaminationrenal cortexratmicrodistributionimaging
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 1 , February 2012 , pp. 123 - 133
Copyright
Copyright © Microscopy Society of America 2012

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References

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