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Vascular Regression In Gill Filters of Tadpoles of Xenopus Laevis Daudin: A Qualitative and Quantitative Sem Study on Vascular Corrosion Casts

Published online by Cambridge University Press:  02 July 2020

H. Bartel ,
B. Minnich ,
I. Margreiter  and
A. Lametschwandtner
H. Bartel
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(, Europe)
B. Minnich
Affiliation:
Department of Electron Microscopy, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(, Europe)
I. Margreiter
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(, Europe)
A. Lametschwandtner
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(, Europe)
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Extract

During metamorphosis amphibian tadpoles regress gills, tail and other larval structures. Thus gills are a good model to study how a highly complex three-dimensional vascular network regresses and finally disappears. Presently, only the microvascularization of external gills of larvae of Hynobiusdunni(2) and Litoria ewingi(3) is examined. The present study analyzes the spatio-temporal changes which the gill 3D-vascular network undergoes during metamorphosis using SEM of microvascular corrosion castings and a newly developed 3D-morphometry system (4, 5).

Vascular casts of gills of tadpoles of the South African Clawed Toad, Xenopus laevis Daudin of stages 57 to 62 (6) were analyzed by SEM. Stereopaired images (tilting angle: 6 degrees) grabbed with a slow scan frame grabber (Orion 4.27) from the SEMs photo-display were imported into a 3Dmorphometry system (6) to measure vessel diameters, interbranching distances, branching angles and branching orders of filter row venules. This study reports the changes in the branching orders only.

Type
Applications and Advances In Vascular Corrosion Casting In Microvascular Research
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1188 - 1189
Copyright
Copyright © Microscopy Society of America

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References

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5. Malkusch, W. et al., Anal. Cell. Pathol. 9, (1995) 69–81Google Scholar

6. Minnich, B.. Doctoral Thesis, University of Salzburg, 1998Google Scholar