This tutorial emphasizes the fundamentals of the use of light microscopy in combination with electronic imaging. The goal of the tutorial is to enable the attendees to make informed choices both about the modes of imaging best suited to their application and about the most suitable components to be employed. The didactic portion of the program will consist of five presentations, each dealing with different aspects of modern imaging systems and their applications to problems of biological significance. A laboratory demonstration session, organized to complement the lectures will follow.

The morning session will begin will a brief review by Dr. Spring of the principles of light detection and the formation of the video signal. Dr. Salmon will then describe video-enhanced contrast microscopy. This transmitted light imaging technique, widely utilized to visualize low contrast specimens exploits the wide dynamic range and high gain of video cameras for the capture of images that are virtually invisible through the eyepieces because of high background light.