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Alterations in the Fat Body Cells of Rhinocricus padbergi (Diplopoda) Resulting from Exposure to Substrate Containing Sewage Sludge

Published online by Cambridge University Press:  08 February 2012

Raphael Bastão de Souza  and
Carmem Silvia Fontanetti
Raphael Bastão de Souza
Affiliation:
Department of Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24A, 1515, 13506-900, Rio Claro, SP, Brazil
Carmem Silvia Fontanetti*
Affiliation:
Department of Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24A, 1515, 13506-900, Rio Claro, SP, Brazil
*
Corresponding author. E-mail: fontanet@rc.unesp.br
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Abstract

The final disposal of residues generated at sewage treatment plants (STPs) has become a major problem for cities, due to the increase in the amount of treated sewage. One of the alternatives for the residue, labeled “sewage sludge,” is its reuse in agriculture and in degraded soil. However, not all pathogens and metals present in it are eliminated during treatment. Diplopods have been used as bioindicators in ecotoxicological tests as they are constantly in close contact with the soil. Owing to this fact, the purpose of this study was to expose specimens of the diplopod Rhinocricus padbergi to substrate containing sewage sludge collected at STPs to analyze morphological alterations in their parietal and perivisceral fat body, where substances are stored. The exposures were held for 7, 15, or 90 days at different concentrations of sewage sludge (control, 1%, 10%, and 50%). The parietal fat body showed no alterations in any of the three exposure periods or concentrations. Alterations in the perivisceral fat body were observed for all exposure periods. According to the results, we suggest that the sludge used has toxic agents responsible for changing the animal's perivisceral fat body.

Keywords

soil toxicitySTPhistochemistryhistologyacute exposuresubchronic exposuremillepede
Type
Biological and Biomedical Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 2 , April 2012 , pp. 317 - 323
Copyright
Copyright © Microscopy Society of America 2012

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