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Application of Cryo-Electron Microscopy on Drug Discovery

Published online by Cambridge University Press:  30 July 2021

Viswanath Vittaladevaram  and
Kranthi Kuruti
Viswanath Vittaladevaram
Affiliation:
BIODISCOVERY GROUP, Anantapur, India
Kranthi Kuruti
Affiliation:
Mahindra India, Tirupathi, India

Abstract

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The key aspect for development of novel drug molecules is to perform structural determination of target molecule associated with its ligand. One such tool that provides insights towards structure of molecule is Cryo-electron microscopy which covers biological targets that are intractable. Examination of proteins can be carried out in native state, as the samples are frozen at -175 degree Celsius i.e. cryogenic temperatures. In addition to this, there were no limits for molecular and functional structures of proteins that can be imagined in 3-dimensional form. This includes ligands which unravel mechanisms that are biologically relevant. This will enable to better understand the mechanisms that are used for development of new therapeutics. Application of Cryo-electron microscopy is not limited to protein complexes and is considered as non-specific. Intervention of Cryo-EM would allow to analyse the structures and also able to dissect the interaction with therapeutic molecules. The study determines the usage of cryo-EM for providing resolutions that are acceptable for lead discovery. It also provides support for lead optimization and also for discovery of vaccines and therapeutics.

Type
Cryo-EM in Drug Discovery
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 3250
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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