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Characterization of Hydroxyapatite by Electron Microscopy

Published online by Cambridge University Press:  15 November 2005

V. Rodríguez-Lugo ,
J. Sanchez Hernández ,
Ma. J. Arellano-Jimenez ,
P.H. Hernández-Tejeda  and
S. Recillas-Gispert
V. Rodríguez-Lugo
Affiliation:
Centro Universitario de Vinculación, Benemérita Universidad Autónoma de Puebla, 29 Oriente 601-1 Col. Ladrillera de Benítez, C.P. Puebla 72520, México Facultad de Ingeniería Química, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio y 18 sur, Ciudad Universitaria, C.P. 72570 Puebla, México
J. Sanchez Hernández
Affiliation:
Centro Universitario de Vinculación, Benemérita Universidad Autónoma de Puebla, 29 Oriente 601-1 Col. Ladrillera de Benítez, C.P. Puebla 72520, México
Ma. J. Arellano-Jimenez
Affiliation:
Instituto de Física, Universidad Nacional Autónoma de México, A.P. 1-1010, Querétaro, 76000, México
P.H. Hernández-Tejeda
Affiliation:
Instituto de Física de la Benemérita Universidad Autónoma de Puebla, Apdo. Postal J-48 Puebla 72520, México
S. Recillas-Gispert
Affiliation:
Centro Universitario de Vinculación, Benemérita Universidad Autónoma de Puebla, 29 Oriente 601-1 Col. Ladrillera de Benítez, C.P. Puebla 72520, México Facultad de Ingeniería Química, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio y 18 sur, Ciudad Universitaria, C.P. 72570 Puebla, México
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Abstract

The obtention of hydroxyapatite (HAp) is reported using brushite (CaHPO4·2H2O) and the skeleton of a starfish (Mellita eduardobarrosoi sp. nov.), primarily composed of magnesian calcite ((Ca,Mg)CO3) as precursors. Stoichiometric amounts of both were reacted under hydrothermal conditions: a pressure of 5.8 MPa and a temperature of 200°C for 2, 4, 6, 8, 10, and 20 h of reaction times. The samples obtained were characterized by means of scanning electron microscopy, X-ray diffraction, infrared spectroscopy, and transmission electron microscopy. Two defined populations of HAp fibers were found: A bundle of fibers 75 μm in length and 1–13 μm in diameter, and a second bundle of fibers 5 μm in length and less than 0.5 μm in diameter. Furthermore, an increase in HAp formation and a Ca/P ratio as a function of reaction time were observed. The growth mechanism of HAp is also discussed.

Keywords

brushitediffraction and spectroscopyelectron microscopyhydroxyapatitestarfish
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 11 , Issue 6 , December 2005 , pp. 516 - 523
Copyright
© 2005 Microscopy Society of America

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References

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