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Cryo-Electron Microscopy of Potassium Channel Membrane Proteins

Published online by Cambridge University Press:  27 August 2014

Julia Kowal ,
Sebastian Scherer ,
Kushal Sejwal ,
Mohamed Chami ,
Paul Baumgartner ,
Martina Rangl ,
Simon Scheuring ,
Gunnar F. Schröder ,
Crina Nimigean  and
Henning Stahlberg
Julia Kowal
Affiliation:
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland
Sebastian Scherer
Affiliation:
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland
Kushal Sejwal
Affiliation:
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland
Mohamed Chami
Affiliation:
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland
Paul Baumgartner
Affiliation:
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland
Martina Rangl
Affiliation:
U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, 13009 Marseille, France
Simon Scheuring
Affiliation:
U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, 13009 Marseille, France
Gunnar F. Schröder
Affiliation:
Forschungszentrum Jülich, Institute of Complex Systems, ICS-6: Structural Biochemistry, 52425 Jülich; and Department of Physics, Heinrich-Heine University Düsseldorf, 40225 Düsseldorf, Germany
Crina Nimigean
Affiliation:
Departments of Anesthesiology, Physiology and Biophysics, and Biochemistry, Weill Cornell Medical College, 1300 York Ave, New York, New York 10065, USA
Henning Stahlberg
Affiliation:
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 20 , Supplement S3: Proceedings of Microscopy & Microanalysis 2014 , August 2014 , pp. 1206 - 1207
Copyright
Copyright © Microscopy Society of America 2014 

References

[1] Kowal, , et al, Nat Commun (2014). Jan 28;5:3106.Google Scholar
[2] Scherer, , et al, J Struct Biol (2013). Dec 29. pii: S1047-8477(13)00338-9.Google Scholar
[3] Scherer, , et al, 2dx_automator: Implementation of a Semiautomatic High-Throughput High-Resolution Cryo-Electron Crystallography Pipeline (2014). Submitted.Google Scholar
[4] This work was supported by the Swiss National Science Foundation (SNF 315230_127545, National Centers for Competence in Research, Structural Biology and TransCure), the Swiss Initiative for Systems Biology (SystemsX.ch), the National Institutes of Health (NIH GM077560 to C.M.N.), the European Research Council (Grant #310080 to S.S.), and Agence National de la Recherche (ANR, Grants # ANR-12-BS10-009-01 and ANR-12-BSV8-0006-01 to S.S.). We acknowledge expert technical assistance of B. Anderson and K. Goldie. We thank S.A. Müller for insightful discussions and manuscript editing.Google Scholar