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Distribution of Lignin, Hemicellulose, and Arabinogalactan Protein in Hemp Phloem Fibers

Part of: Micrographia Collection

Published online by Cambridge University Press:  03 September 2018

Shingo Kiyoto ,
Arata Yoshinaga ,
Eva Fernandez-Tendero ,
Arnaud Day ,
Brigitte Chabbert  and
Keiji Takabe
Shingo Kiyoto*
Affiliation:
Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Uji, Kyoto 611-0011, Japan
Arata Yoshinaga
Affiliation:
Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
Eva Fernandez-Tendero
Affiliation:
FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 51100 Reims, France Fibres Recherche Développement, Technopole de l’Aube en Champagne – Hôtel de Bureaux 2, 2 rue Gustave Eiffel, CS 90601, 10901 TROYES, Cedex 9, France CNRS, UMR8576, UGSF- Unité de Glycobiologie Structurale et Fonctionnelle, Unité de Lille, 59000 Lille, France
Arnaud Day
Affiliation:
Fibres Recherche Développement, Technopole de l’Aube en Champagne – Hôtel de Bureaux 2, 2 rue Gustave Eiffel, CS 90601, 10901 TROYES, Cedex 9, France CNRS, UMR8576, UGSF- Unité de Glycobiologie Structurale et Fonctionnelle, Unité de Lille, 59000 Lille, France
Brigitte Chabbert
Affiliation:
FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 51100 Reims, France
Keiji Takabe
Affiliation:
Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
*
*Author for correspondence: Shingo Kiyoto, E-mail: shingokiyoto262382410@yahoo.co.jp
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Abstract

The distribution of lignin, 8-5′ and 8-8′ linked lignin substructure, and noncellulosic polysaccharides in hemp (Cannabis sativa L.) phloem fibers were explored based on histochemical and immunological methods. Ultraviolet absorption and potassium permanganate staining were observed mainly in the compound middle lamella (CML) and S1 layers, and rarely in the G-layer of phloem fibers, suggesting that lignin concentration is high at the CML and S1 layers, and very low at the G-layer of hemp fibers. Acriflavine staining, uniform KM1 labeling (8-5′ linked lignin substructure), and no KM2 labeling (8-8′ linked structure) were observed in the G-layer, suggesting that there is a small amount of lignin-like compound with 8-5′ linked structure in the G-layer. In addition, some fiber cells showed a multilayered structure. Uniform arabinogalactan protein (AGP) labeling was observed on the S1 layers and G-layers using JIM14, but little appeared in the CML of hemp fibers, indicating that these layers of the phloem fibers contain AGP. Immunogold labeling of xylan (LM11) and glucomannan (LM21) showed that xylan and glucomannan were mainly present in the S1 layers and the G-layers, respectively. In some phloem fibers, LM21 immunofluorescence labeling showed multilayered structure, suggesting the heterogeneous distribution of glucomannan.

Keywords

Cannabis sativa L.lignin8-5′ linkage8-8′ linkagemonoclonal antibody
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 24 , Issue 4 , August 2018 , pp. 442 - 452
Copyright
© Microscopy Society of America 2018 

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