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Feline HER2 Protein Expression Levels and Gene Status in Feline Mammary Carcinoma: Optimization of Immunohistochemistry (IHC) and In Situ Hybridization (ISH) Techniques

Published online by Cambridge University Press:  30 May 2013

Maria Soares ,
Jorge Correia ,
Pedro Rodrigues ,
Margarida Simões ,
Alves de Matos  and
Fernando Ferreira
Maria Soares
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
Jorge Correia
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
Pedro Rodrigues
Affiliation:
Escola Superior de Saúde Egas Moniz, Quinta da Granja, Monte da Caparica, 2829-511 Caparica, Portugal
Margarida Simões
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
Alves de Matos
Affiliation:
Escola Superior de Saúde Egas Moniz, Quinta da Granja, Monte da Caparica, 2829-511 Caparica, Portugal Serviço de Anatomia Patológica, Hospital Curry Cabral, Centro Hospitalar de Lisboa Central, R. da Beneficiência, 1069-166 Lisboa, Portugal
Fernando Ferreira*
Affiliation:
CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
*
*Corresponding author. E-mail: fernandof@fmv.utl.pt
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Abstract

Human epidermal growth factor receptor (HER2) is a tumor biomarker that when overexpressed and/or amplified is associated with a poor prognosis for women with breast cancer. This specific tumor subtype is eligible for a specific immunotherapy that increases survival period. However, in feline oncology, only a few studies have been performed on molecular characterization of feline (fHER2) in feline mammary carcinoma (FMC), and the available data are inconsistent. In this study, fHER2 protein levels and gene status in FMC were evaluated by immunohistochemistry and in situ hybridization. After being optimized, these techniques revealed that fHER2 is overexpressed in 33% of FMC cases, although fHER2 and fTOP2A gene amplification could not be observed. Our results support the possibility of using FMC as a natural model for comparative oncology. Additional data obtained may also improve the diagnostics, and consequently the treatment, of this type of tumor in veterinary medicine.

Keywords

feline mammary carcinomas (FMC)feline human epidermal growth factor receptor-2 gene homolog (fHER2)feline topoisomerase 2 alpha gene homolog (fTOP2A)gene amplificationprotein overexpressionimmunohistochemistry (IHC)in situ hybridization (ISH)
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 4 , August 2013 , pp. 876 - 882
Copyright
Copyright © Microscopy Society of America 2013 

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