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Iridovirus-Like Viruses in Erythrocytes of Lacertids from Portugal

Published online by Cambridge University Press:  28 June 2013

António P. Alves de Matos ,
M. Filomena Caeiro ,
Filipa F. Vale ,
Eduardo Crespo  and
Ilan Paperna
António P. Alves de Matos*
Affiliation:
Centro de Estudos do Ambiente e do Mar (CESAM/FCUL), Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Quinta da Granja, Monte de Caparica, 2829-511 Caparica, Portugal
M. Filomena Caeiro
Affiliation:
Centro de Estudos do Ambiente e do Mar (CESAM/FCUL), Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal
Filipa F. Vale
Affiliation:
Centro de Estudos do Ambiente e do Mar (CESAM/FCUL), Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal Faculdade de Engenharia, Universidade Católica Portuguesa, 2635-631 Rio de Mouro, Portugal
Eduardo Crespo
Affiliation:
Centro de Biologia Ambiental da FCUL, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal
Ilan Paperna
Affiliation:
Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences of the Hebrew University of Jerusalem, Rehovot 76100, Israel
*
*Corresponding author. E-mail: apamatos@kanguru.pt
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Abstract

Icosahedral nucleo-cytoplasmic large DNA viruses (NCLDV)-like viruses, which form inclusions in the erythrocyte cytoplasm of reptiles, were previously presented as candidates for a new genus of the Iridoviridae family. The present work describes the distribution of infected lizard hosts and ultrastructural characteristics of the viral inclusions of NCLDV-like viruses from Portugal and adjacent locations in Spain. Giemsa-stained blood smears of 235 Lacerta schreiberi from Portugal and Spain, 571 Lacerta monticola from the mountain Serra da Estrela (Portugal), 794 Podarcis hispanica from several localities in Portugal and Spain, and 25 Lacerta dugesii from Madeira Island, were studied. Infection in L. schreiberi was only found in mountain populations, up to 30% in Serra da Estrela and 9–11% elsewhere. It was absent in lizards from lowlands. Prevalence of infection among L. monticola in Serra da Estrela was 10%; infected lizards were found during March to July and October but not in August and September. Infection in P. hispanica was below 3.3%. Only one infected specimen of L. dugesii was identified by light microscopy. Ultrastructural examination of infected samples revealed that the inclusions are virus assembly sites of icosahedral cytoplasmic iridovirus-like virions. Virions from different host species have different ultrastructural features and probably represent different related viruses.

Keywords

lizard erythrocytic virusesIridovirus-likeultrastructureLacerta spp.PodarcisPortugalSpain
Type
Portuguese Society for Microscopy
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1183 - 1189
Copyright
Copyright © Microscopy Society of America 2013 

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