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Is the Binding Pattern of Zinc(II) Equal in Different Bryophyte Species?

Part of: Micrographia Collection

Published online by Cambridge University Press:  27 February 2018

Marko S. Sabovljević ,
Marieluise Weidinger ,
Aneta D. Sabovljević ,
Wolfram Adlassnig  and
Ingeborg Lang
Marko S. Sabovljević*
Affiliation:
Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
Marieluise Weidinger
Affiliation:
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
Aneta D. Sabovljević
Affiliation:
Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
Wolfram Adlassnig
Affiliation:
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
Ingeborg Lang
Affiliation:
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
*
Author for correspondence: Marko S. Sabovljević, E-mail: marko@bio.bg.ac.rs
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Abstract

Bryophytes are usually taken as good bioindicators. However, they represent a large group of terrestrial plants and they express an enormous range of peculiarities within the plant kingdom. With the aim to search for a common pattern of zinc binding, we established axenical in vitro cultures of a dozen bryophyte species that include hornworts, thallose, and leafy liverworts, as well as acrocarp and pleurocarp mosses. The species were grown free of contaminants for many years prior to the application of different treatments, i.e. offering Zn(II) from solid and liquid media and in combination with different anions. The localization and binding of zinc was detected by confocal microscopy using the zinc-specific dye FluoZin™-3. In one of the species, Hypnum cupressiforme (which is widely used for atmospheric heavy metal deposition studies in biomonitoring), semi-quantitative analyses of zinc were performed by energy dispersive X-ray microspectrometry (EDX) in a scanning electron microscope. The results suggest no common pattern of Zn(II) binding in different bryophyte species. Instead, the binding pattern seems to be species specific. Zinc is located in certain areas or cellular compartments, as clearly shown by the EDX measurements in H. cupressiforme.

Keywords

bryophyteszincuptakeconfocal microscopyX-ray microanalysis
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 24 , Issue 1 , February 2018 , pp. 69 - 74
Copyright
© Microscopy Society of America 2018 

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