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Limiting Factors on Image Quality in Imaging through Turbid Media under Single-photon and Two-photon Excitation

Published online by Cambridge University Press:  08 August 2002

S. P. Schilders  and
M. Gu
S. P. Schilders
Affiliation:
Optoelectronic Imaging Group, School of Communications and Informatics, Victoria University of Technology, Australia
M. Gu
Affiliation:
Optoelectronic Imaging Group, School of Communications and Informatics, Victoria University of Technology, Australia
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Abstract

The effect of multiple scattering in a turbid medium on single-photon and two-photon fluorescence microscopy is experimentally investigated for different scattering characteristics including scattering anisotrophy and optical thickness of a turbid medium. It is demonstrated that two-photon excitation can provide significant improvement in penetration depth through turbid media, due to reduced scattering experienced by the excitation beam. It is also shown that the limiting factor in obtaining high-quality images under singlephoton excitation is the fast degradation of image resolution caused by multiple scattering, while for twophoton excitation it is limited by the degradation of image contrast due to the reduction in fluorescence strength.

Type
Articles
Information
Microscopy and Microanalysis , Volume 6 , Issue 2 , March 2000 , pp. 156 - 160
Copyright
2000 Microscopy Society of America

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