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Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice (Mus musculus)

Part of: Micrographia Collection

Published online by Cambridge University Press:  05 March 2020

Ebtsam Al-Olayan ,
Maha Elamin ,
Eman Alshehri ,
Abeer Aloufi ,
Zainab Alanazi ,
Mina Almayouf ,
Lamia Bakr  and
Rewaida Abdel-Gaber Open the ORCID record for Rewaida Abdel-Gaber [Opens in a new window]
Ebtsam Al-Olayan
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Maha Elamin
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Eman Alshehri
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Abeer Aloufi
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Research Chair of Vaccines, Deanship of Scientific Research, King Saud University, Riyadh, Saudi Arabia
Zainab Alanazi
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Mina Almayouf
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Lamia Bakr
Affiliation:
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt
Rewaida Abdel-Gaber*
Affiliation:
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt
*
*Author for correspondence: Rewaida Abdel-Gaber, E-mail: rewaida@sci.cu.edu.eg, eolayan@ksu.edu.sa
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Abstract

Hymenolepis nana, typically a parasite found in conventionally established mouse colonies, has zoonotic potential characterized by autoinfection and direct life cycle. The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide cestode infected 40% of the 50 mice sampled. The rate of infection in males (52%) was higher than in females (28%). Morphological studies on the cestode parasite showed that worms had a globular scolex with four suckers, a retractable rostellum with 20–30 hooks, and a short unsegmented neck. In addition, the remaining strobila consisted of immature, mature, and gravid proglottids, irregularly alternating genital pores, lobulated ovaries, postovarian vitelline glands, and uteri with up to 200 eggs in their gravid proglottids. The parasite taxonomy was confirmed by using molecular characterization based on the sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (mtCOX1) gene. The parasite recovered was up to 80% identical to other species in GenBank. High blast scores and low divergence were noted between the isolated parasite and previously described H. nana (gb| AP017666.1). The phylogenetic analysis using the COX1 sequence places this hymenolepidid species of the order Cyclophyllidea.

Keywords

Hymenolepis specieslaboratory animalsmolecular analysesmorphological characterizationrodents
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 26 , Issue 2 , April 2020 , pp. 348 - 362
Copyright
Copyright © Microscopy Society of America 2020

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