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Optical Tweezers: A Practical Guide

Published online by Cambridge University Press:  08 August 2003

Scot C. Kuo
Affiliation:
Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205
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Abstract

Optical tweezers, or the single-beam optical gradient force trap, is becoming a major tool in biology for noninvasive micromanipulation on an optical microscope. The principles and practical aspects that influence construction are presented in an introductory primer. Quantitative theories are also reviewed but have yet to supplant user calibration. Various biological applications are summarized, including recent quantitative force and displacement measurements. Finally, tantalizing developments for new, nonimaging microscopy techniques based on optical tweezers are included.

Type
Research Article
Copyright
© 1995 Microscopy Society of America

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