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Phagocytosis of PLGA Microparticles in Rat Peritoneal Exudate Cells: A Time-Dependent Study

Published online by Cambridge University Press:  30 May 2006

Anderson de Jesus Gomes ,
Claure Nain Lunardi ,
Flávio Henrique Caetano ,
Laurelúcia Orive Lunardi  and
Antonio Eduardo da Hora Machado
Anderson de Jesus Gomes
Affiliation:
Laboratório de Fotoquímica, Instituto de Química, Universidade Federal de Uberlândia, P.O. Box 593, CEP 38400-089 Uberlândia, MG, Brazil
Claure Nain Lunardi
Affiliation:
Laboratório de Farmacologia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto—USP, Av. Café s/n CEP 14040-903 Ribeirão Preto, SP, Brazil
Flávio Henrique Caetano
Affiliation:
Instituto de Biociências, Universidade Estadual Júlio de Mesquita Filho, Av: 24 N1515 CEP 13506-900 Rio Claro, SP, Brazil
Laurelúcia Orive Lunardi
Affiliation:
Instituto de Biociências, Universidade Estadual Júlio de Mesquita Filho, Av: 24 N1515 CEP 13506-900 Rio Claro, SP, Brazil
Antonio Eduardo da Hora Machado
Affiliation:
Laboratório de Fotoquímica, Instituto de Química, Universidade Federal de Uberlândia, P.O. Box 593, CEP 38400-089 Uberlândia, MG, Brazil
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Abstract

With the purpose of enhancing the efficacy of microparticle-encapsulated therapeutic agents, in this study we evaluated the phagocytic ability of rat peritoneal exudate cells and the preferential location of poly(d,l-lactide-co-glycolic acid) (PLGA) microparticles inside these cells. The microparticles used were produced by a solvent evaporation method and were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Size distribution analysis using DLS and SEM showed that the particles were spherical, with diameters falling between 0.5 and 1.5 μm. Results from cell adhesion by SEM assay, indicated that the PLGA microparticles are not toxic to cells and do not cause any distinct damage to them as confirmed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. Among the large variety of cell populations found in the peritoneal exudates (neutrophils, eosinophils, monocytes, and macrophages), TEM showed that only the latter phagocytosed PLGA microparticles, in a time-dependent manner. The results obtained indicate that the microparticles studied show merits as possible carriers of drugs for intracellular delivery.

Keywords

microparticlesdrug deliveryperitoneal exudate cellsmacrophageTEMSEM
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 12 , Issue 5 , October 2006 , pp. 399 - 405
Copyright
© 2006 Microscopy Society of America

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