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Two-Color Fixed Cell Imaging Using Engineered Point Spread Functions – XPSF Family

Published online by Cambridge University Press:  22 July 2022

Sanduni Fernando ,
Jason Martineau ,
Rob Hobson ,
Erik Jorgensen  and
Jordan Gerton
Sanduni Fernando
Affiliation:
Department of Physics & Astronomy, University of Utah, Salt Lake City, Utah, USA
Jason Martineau
Affiliation:
Department of Physics & Astronomy, University of Utah, Salt Lake City, Utah, USA
Rob Hobson
Affiliation:
Department of Biology, University of Utah, Salt Lake City, Utah, USA
Erik Jorgensen
Affiliation:
Department of Biology, University of Utah, Salt Lake City, Utah, USA
Jordan Gerton*
Affiliation:
Department of Physics & Astronomy, University of Utah, Salt Lake City, Utah, USA
*
*Corresponding author: jgerton@physics.utah.edu

Abstract

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Type
Development, Challenges and Biomedical Applications of Tissue Clearing, Expansion Microscopy and Volumetric Imaging
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1564 - 1566
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Copyright
Copyright © Microscopy Society of America 2022

References

Shechtman, Y., Weiss, L., Backer, A. et al. Multicolour localization microscopy by point-spread-function engineering. Nature Photon 10, 590–594 (2016). https://doi.org/10.1038/nphoton.2016.137CrossRefGoogle ScholarPubMed
Fernando, S., Martineau, J., Jorgensen, E., & Gerton, J. (2021). Optimizing Point Spread Functions to Discern Highly Overlapping Emission Spectra. Microscopy and Microanalysis, 27(S1), 864-867. doi:10.1017/S1431927621003378CrossRefGoogle Scholar
Martineau, J., Jorgensen, E., Menon, R., & Gerton, J. (2018). Precisely Localizing Wavelength Sensitive Point-Spread Functions Engineered With a Silicon Oxide Phase Plate. Microscopy and Microanalysis, 24(S1), 1364-1365. doi:10.1017/S1431927618007304CrossRefGoogle Scholar
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The authors acknowledge funding from Howard Hughes Medical Institute (HHMI) and University of UtahGoogle Scholar