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Two-Color Fixed Cell Imaging Using Engineered Point Spread Functions – XPSF Family

Published online by Cambridge University Press:  22 July 2022

Sanduni Fernando ,
Jason Martineau ,
Rob Hobson ,
Erik Jorgensen  and
Jordan Gerton
Sanduni Fernando
Affiliation:
Department of Physics & Astronomy, University of Utah, Salt Lake City, Utah, USA
Jason Martineau
Affiliation:
Department of Physics & Astronomy, University of Utah, Salt Lake City, Utah, USA
Rob Hobson
Affiliation:
Department of Biology, University of Utah, Salt Lake City, Utah, USA
Erik Jorgensen
Affiliation:
Department of Biology, University of Utah, Salt Lake City, Utah, USA
Jordan Gerton*
Affiliation:
Department of Physics & Astronomy, University of Utah, Salt Lake City, Utah, USA
*
*Corresponding author: jgerton@physics.utah.edu

Abstract

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Type
Development, Challenges and Biomedical Applications of Tissue Clearing, Expansion Microscopy and Volumetric Imaging
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1564 - 1566
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Copyright
Copyright © Microscopy Society of America 2022

References

Shechtman, Y., Weiss, L., Backer, A. et al. Multicolour localization microscopy by point-spread-function engineering. Nature Photon 10, 590–594 (2016). https://doi.org/10.1038/nphoton.2016.137CrossRefGoogle ScholarPubMed
Fernando, S., Martineau, J., Jorgensen, E., & Gerton, J. (2021). Optimizing Point Spread Functions to Discern Highly Overlapping Emission Spectra. Microscopy and Microanalysis, 27(S1), 864-867. doi:10.1017/S1431927621003378CrossRefGoogle Scholar
Martineau, J., Jorgensen, E., Menon, R., & Gerton, J. (2018). Precisely Localizing Wavelength Sensitive Point-Spread Functions Engineered With a Silicon Oxide Phase Plate. Microscopy and Microanalysis, 24(S1), 1364-1365. doi:10.1017/S1431927618007304CrossRefGoogle Scholar
Kirshner, H., Aguet, F., Sage, D., and Unser, M., J. Microsc. 249, 13 (2013)10.1111/j.1365-2818.2012.03675.xCrossRefGoogle Scholar
Juette, M. F., Gould, T. J., Lessard, M. D., Mlodzianoski, M. J., Nagpure, B. S., Bennett, B. T., Hess, S. T., and Bewersdorf, J., Nat Meth 5, 527 (2008)10.1038/nmeth.1211CrossRefGoogle Scholar
The authors acknowledge funding from Howard Hughes Medical Institute (HHMI) and University of UtahGoogle Scholar