Hostname: page-component-84b7d79bbc-7nlkj Total loading time: 0 Render date: 2024-07-28T09:22:49.392Z Has data issue: false hasContentIssue false
  • English
  • Français

Using Maskless Photopatterning for Cryo-ET of Primary Drosophila Melanogaster Neurons

Published online by Cambridge University Press:  30 July 2021

Joseph Kim ,
Bryan Sibert ,
Jae Yang ,
Sihui Yang ,
Josephine Mitchell ,
Jill Wildonger  and
Elizabeth Wright
Joseph Kim
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Bryan Sibert
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Jae Yang
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Sihui Yang
Affiliation:
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, United States
Josephine Mitchell
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Jill Wildonger
Affiliation:
Department of Biological Sciences, University of California, San Diego, La Jolla, California, United States
Elizabeth Wright
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Cryo-electron Tomography: Present Capabilities and Future Potential
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 2818 - 2821
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Wagner, J. et al. Cryo-electron tomography-the cell biology that came in from the cold. FEBS Lett. 591, 2520-2533 (2017).Google ScholarPubMed
Tao, C.-L. et al. Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy. J. Neurosci. 38, 1493–1510 (2018).CrossRefGoogle ScholarPubMed
Guo, Q. et al. In Situ Structure of Neuronal C9orf72 Poly-GA Aggregates Reveals Proteasome Recruitment. Cell. 172, 696-705 (2018).Google ScholarPubMed
Fischer, T. D. et al. Morphology of mitochondria in spatially restricted axons revealed by cryo-electron tomography. PLOS Biology 16 (2018).CrossRefGoogle ScholarPubMed
Lucic, V. et al. Multiscale imaging of neurons grown in culture: From light microscopy to cryo-electron tomography. J. Struct Biol. 160, 146–156 (2007).CrossRefGoogle ScholarPubMed
Toro-Nahuelpan, M. et al. Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies. Nat Methods. 17, 50–54 (2020).CrossRefGoogle ScholarPubMed
Engel, L. et al. Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies. J. Micromech Microeng. 29 (2019).CrossRefGoogle ScholarPubMed
Kim, J. et al. A New In Situ Neuronal Model for Cryo-ET. Microsc. Microanal. 26, 130-132 (2020).CrossRefGoogle Scholar
Schrod, N. et al. Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons. PLOS ONE 13 (2018).CrossRefGoogle ScholarPubMed
Atherton, J. et al. Microtubule architecture in vitro and in cells revealed by cryo-electron tomography. Acta Crystallogr D Struct Biol. 74, 572-584 (2018).CrossRefGoogle ScholarPubMed
Duffy, J. B. GAL4 system in Drosophila: a fly geneticist's Swiss army knife. Genesis (2002).Google ScholarPubMed
Czöndör, K. et al. Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation. Nat Comm. 4 (2013).Google ScholarPubMed
This research was supported by funds from the University of Wisconsin-Madison, Morgridge Institute for Research, and National Institutes of Health (R01GM104540, R01GM104540-03S1, and U24 GM139168) to E.R.W. All EM data was collected at the Cryo-EM Research Center, Department of Biochemistry, University of Wisconsin-Madison.Google Scholar