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Using Maskless Photopatterning for Cryo-ET of Primary Drosophila Melanogaster Neurons

Published online by Cambridge University Press:  30 July 2021

Joseph Kim ,
Bryan Sibert ,
Jae Yang ,
Sihui Yang ,
Josephine Mitchell ,
Jill Wildonger  and
Elizabeth Wright
Joseph Kim
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Bryan Sibert
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Jae Yang
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Sihui Yang
Affiliation:
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, United States
Josephine Mitchell
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States
Jill Wildonger
Affiliation:
Department of Biological Sciences, University of California, San Diego, La Jolla, California, United States
Elizabeth Wright
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States

Abstract

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Type
Cryo-electron Tomography: Present Capabilities and Future Potential
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 2818 - 2821
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

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This research was supported by funds from the University of Wisconsin-Madison, Morgridge Institute for Research, and National Institutes of Health (R01GM104540, R01GM104540-03S1, and U24 GM139168) to E.R.W. All EM data was collected at the Cryo-EM Research Center, Department of Biochemistry, University of Wisconsin-Madison.Google Scholar