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Published online by Cambridge University Press: 14 March 2018
Since the earliest explorations of the microscopic world in the 17th century and continuing to the present day, microscopists have continuously faced a challenge in that there is an inherent tradeoff between the resolution of the microscope and the size of the field of view--as the resolution of the microscope is increased to resolve smaller features, the observable area of the specimen decreases proportionally. While being a long recognized characteristic of the traditional microscope design, this tradeoff is becoming a significant hindrance as optical microscopes are being used in automated systems for advanced biotech research, medical diagnostics, robotic micromanipulation, and industrial inspection.