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Applications of Focused Ion Beam (FIB) on Yeast Cell & SARS Virus

Published online by Cambridge University Press:  14 March 2018

H. L. Hing ,
C. Burkhardt ,
P. Gnauck ,
S. Sally ,
H. Gelderbloms ,
Y. Muranaka ,
M.A. Kaswandi ,
A.H.A. Azizl  and
A.Z. Sahalan
H. L. Hing
Affiliation:
Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, (National University of Malaysia) Kuala Lumpur
C. Burkhardt
Affiliation:
Natural & Medical Science Institute (NMI) Germany, Reutlingen, Germany
P. Gnauck
Affiliation:
Natural & Medical Science Institute (NMI) Germany, Reutlingen, Germany
S. Sally
Affiliation:
Electron Microscope Unit, Australia National University, Canberra, Australia
H. Gelderbloms
Affiliation:
Robert Koch Institut, Berlin, Germany
Y. Muranaka
Affiliation:
Research Equipment Center, Hamamatsu University School of Medicine, Hamamatsu, Japan
M.A. Kaswandi
Affiliation:
Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, (National University of Malaysia) Kuala Lumpur
A.H.A. Azizl
Affiliation:
Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, (National University of Malaysia) Kuala Lumpur
A.Z. Sahalan
Affiliation:
Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, (National University of Malaysia) Kuala Lumpur

Extract

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The focused ion beam (FIB) is a relatively novel technique to biomedical electron microscopy as it open up new means for the observations and examinations of a wide range of biomedical and biological materials. The focused ion beam, or FIB tool has been utilized mainly in the fields of material sciences and industry. The (FIB) uses high-energy gallium ions to precisely and accurately section or mill samples. Lately FIB method have been used to prepare biological samples such as yeast cells and virus particles. Yeast cells Schwanniomyces occidentalis S. occidentalis were prepared by vacuum sucking them into cellulose tubing, plunge freezing them in liquid nitrogen, followed by chemical fixation in glutaraldehye and postfixed with osmium tetroxide, dehydrated in a series of ascending alcohol concentration up to absolute alcohol, then freeze dried overnight. In the case of SARS virus, the tissue culture containing virus particles was chemically fixed with glutaraldehyde, dehydrated in ascending order of alcohol concentrations and then freeze dried.

Type
Research Article
Information
Microscopy Today , Volume 15 , Issue 5 , September 2007 , pp. 42 - 43
Copyright
Copyright © Microscopy Society of America 2007

References

References:

[1]. Monroe, P.R. & Dickson, M.A. 2001. Proc. of the 10th Scientific Conference Electron Microscopy Soc of Malaysia, Kuala Lumpur, Malaysia (2001): 8.Google Scholar
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[4]. The contributions Dr. Sally Stowe, EM Unit, ANU, Canberra, Australia & Dr. Peter Gnauck of Zeiss, Germany, Ms Normalawati Shamsudin is greatly acknowledged.Google Scholar