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Choosing a Cantilever for In Situ Atomic Force Microscopy

Published online by Cambridge University Press:  14 March 2018

John T. Woodward
John T. Woodward*
Affiliation:
Biotechnology Division, National Institute of Standards and Technology
*
John.Woodward@nist.gov

Extract

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What is the best cantilever for intermittent contact mode (often called Tapping Mode™) atomic force microscope (AFM) imaging under water? This is a question I hear often and one that recently generated some interesting discussion on an AFM newsgroup (more on the newsgroup below). The ability of the AFM to image samples En physiologically relevant environments has made it a popular technique in the biological sciences. However, because scanning the AFM tip in contact mode easily perturbs many biological samples, it was the advent of intermittent contact modes that lead to AFM's widespread use in biology.

Type
Research Article
Information
Microscopy Today , Volume 11 , Issue 2 , April 2003 , pp. 42 - 43
Copyright
Copyright © Microscopy Society of America 2003

References

Endnotes

1 Tapping Mode is a registered trademark of Digital Instruments Corporation.

2 The mention of products in this manuscript is not meant as an endorsement by NIST nor as an indication that they are the best available.

3 A. R French; Vibrations and Waves; W. W. Norton & Co.; New York, 1971; 77- 100.

4 B. Drake et al Science 243,1568-1571 (1969).

5 M. B, Viani et al., Nature Structural Biology, 644-647 (2000).