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HMDS and Specimen Drying for SEM:

Published online by Cambridge University Press:  14 March 2018

Philip Oshel

Extract

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Hexamethyltiisilizane (HMDS) is an excellent method of chemical drying of hydrated specimens. There are several variables involved in its use, the most easily controlled being the number of transitional steps from 100% eihanol (EtOH) to 100% HMDS and the drying temperature.

Fixation and dehydration are the same for both HMDS and CPD. Once thespecimenisinthefinal 100% ethanol, it must then be transferred to 100% HMDS through a graded series of ethanol-HMDS mixtures.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 4 , May 1997 , pp. 16
Copyright
Copyright © Microscopy Society of America 1997