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Hold-'Em In Place

Published online by Cambridge University Press:  14 March 2018

Kristen R. Ross  and
Michael E. Berens
Kristen R. Ross*
Affiliation:
Barrow Neurological Institute
Michael E. Berens*
Affiliation:
Barrow Neurological Institute
*
kross@chw.edu
mberens@CHW.EDU

Extract

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We've used gelled agarose to cover & hold monolayer cells in place when treatment leads to cell lifting. Low-gelling temperature agarose (Type VII) (Sigma A 4018 or A 0701) can be made as a 2% stock solution in “phosphate-buffered saline” (PBS) (boiled then kept at 42° C to remain liquid). The agarose can be stored at room temperature at this time, but must be boiled and melted again before use.

Type
Research Article
Information
Microscopy Today , Volume 9 , Issue 2 , March 2001 , pp. 18 - 21
Copyright
Copyright © Microscopy Society of America 2001