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Microscope Immersion Oil

Published online by Cambridge University Press:  14 March 2018

Robert Sacher
Robert Sacher*
Affiliation:
Cargiile Labs

Extract

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Microscope immersion oil is used in light microscopy to improve imaging. The use of microscope immersion oil as part of a microscope lens system will produce a brighter and sharper image than a similar design not using immersion oil. The reason is that this oil replaces the air gaps between the condenser and the bottom of the slide and between the top of the slide or cover glass and the objective lens with a medium that has a refractive index equal to the lowest refractive index of these glass components.

How does this work? There is a cone of light extending from a centered point on the specimen to the diameter of the objective lens. The angle of this cone is the Angular Aperture (A.A.). A.A. ranges from 10° for long focus low powered objectives to 140° for short focus high power objectives.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 8 , October 2000 , pp. 33 - 35
Copyright
Copyright © Microscopy Society of America 2000

References

1. McCrone, W.C., McCrone, L.B., and Delly, J.G., Polarized Light Microscopy. Microscope Publications, 1984, pp. 1–48 Google Scholar
2. Cargille, J.J., Immersion Oil and the Microscope, Cargille Laboratories, 2d ed. 1985 Google Scholar