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Modified Cryo-Preparation for Studying Salt Glands in the Turf Grass Zoysia matrella

Published online by Cambridge University Press:  14 March 2018

Sheetal Rao ,
Michael W. Pendleton ,
Marla L. Binzel  and
E. Ann Ellis
Sheetal Rao
Affiliation:
Department of Horticulture and Microscopy and Imaging Center, Texas A&M University, College Station, TX
Michael W. Pendleton
Affiliation:
Department of Horticulture and Microscopy and Imaging Center, Texas A&M University, College Station, TX
Marla L. Binzel
Affiliation:
Department of Horticulture and Microscopy and Imaging Center, Texas A&M University, College Station, TX
E. Ann Ellis
Affiliation:
Department of Horticulture and Microscopy and Imaging Center, Texas A&M University, College Station, TX

Extract

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Zoysia, a common turf grass, is characterized by the presence of functional salt glands. These glands are specialized structures through which the plants excrete excess salt. Research on the mechanism of salt secretion in Zoysia matrella (Manila grass) prompted the development of a specimen preparation technique that would preserve the secreted salt and salt gland. Conventional aqueous preparative techniques wash away the secreted salt on the leaf surface. A specimen preparation technique was modified from a simple cryo-preparative technique for examining hydrogels in the transmission electron microscope.

Type
Research Article
Information
Microscopy Today , Volume 16 , Issue 2 , March 2008 , pp. 44 - 45
Copyright
Copyright © Microscopy Society of America 2008

References

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