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Pioloform F Instead of Formvar as a Support Film for TEM

Published online by Cambridge University Press:  14 March 2018

Victoria J. Madden
Victoria J. Madden*
Affiliation:
University of North Carolina – Chapel Hill

Extract

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Pioloform F (Wacker Chemie Co., Munich, Germany) is an excellent plastic for use as a support film for electron microscopy. The advantages of Pioloform F are high mechanical and thermal stability, and low material bulk (Stockem, 1970). Because of these properties, thinner films than Formvar or collodion may be used and remain stable under the electron beam without subsequent stabilization by carton coating. Thin support films of gray interference color are prepared by dissolving 0.5 grams of Pioloform F powder in 100 milliliters of anhydrous chloroform (Stockem, 1970), using the dip method described by Reimer (1967).

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 10 , December 1998 , pp. 22 - 23
Copyright
Copyright © Microscopy Society of America 1998

References

Behnke, O., Ammittzboll, T., Jessen, H., Klokker, M., Nilausen, K., J., Google Scholar
Tranum-Jensen, , Olsson, L.. 1986. Non-specific binding of protein-stabilized gold sols as a source of error in immunotytochemisiry. Eur. J. Cell. Biol. 41:326.Google Scholar
Birrell, G.B., Hedberg, K.K., and Griffith, O.H., 1987. Pitfalls of immunogold labeling: Analysis of light microscopy, transmission eledron microscopy, and photoelectron microscopy. J. Histacheni. Cytochem. 35: 843-53.CrossRefGoogle ScholarPubMed
Hayat, M.A. and Miller, S.E., 1990. Negative Staining. McGraw-Hill Publishing Company, New York, 215–224.Google Scholar
Reimer, L 1967. Elektronenmikroskopische Untersuchungs- und Prapara tions-mothoden 2. Aufl. Spring-Verlag, Berlin.CrossRefGoogle Scholar
Stockem, W. 1970. The suitability of Pioloform F for the preparing of electron microscopic supporting films. Mikrostopia 26 (516):185–189.Google Scholar