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Preventing Nuclear Artifact in Hematologic Specimens

Published online by Cambridge University Press:  14 March 2018

Robert Santoianni
Robert Santoianni*
Affiliation:
Emory University Hospital, Atlanta, Georgia

Abstract

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The bubbled, popcorn or moth-eaten appearance of nuclei hampered accurate microscopic evaluation of bone marrow and lymph node biopsies. This artifact was also noticed in skin biopsies, but had little bearing on diagnosis. All aspects of specimen handling processing and staining were scrutinized and improved morphological detail was attained only after blocks were recut and allowed to dry completely before staining.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 4 , May 1998 , pp. 16
Copyright
Copyright © Microscopy Society of America 1998

References

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2. Berard, C., Bowling, M.; Technical Factors in Evaluation of Lymph Node Biopsies, Tutorial on Neoplastic Hematopathology, Universal City, CA, February 1989.Google Scholar
3. Beard, C., Nabers, K., Bowling, M., Berard, C.: Achieving Technical Excellence in Lymph Node Specimens; An Update. Laboratory Medicine 16:468–475, 1985.CrossRefGoogle Scholar