Hostname: page-component-77c89778f8-9q27g Total loading time: 0 Render date: 2024-07-21T12:25:30.729Z Has data issue: false hasContentIssue false

Quantitation In Immunohistochemistry

Published online by Cambridge University Press:  14 March 2018

Barry R. J. Rittman*
Affiliation:
The University Of Texas

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Valuable information concerning the relative amounts of the end proucts of histochemical and immunochemical reactions present in sections may be provided by qualitative evaluations, however, greater reliance is often placed on quantitative evaluations. Many quantitative evaluations are based on the use of image analysis and optical density readings of the visible end products. An important question is whether these quantitative measurements are reliable, accurate and reproducible, and if quantitation of these reactions offers any real advantage over qualitative evaluations.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 1998

References

Burdick, HE. 1997. Digital Imaging; theory and applications. McGraw Hill.Google Scholar
Elias, H., Hennig, A., and Schwarz, DE. (1971) Stereology: Applications to biomedical research. Physiological Reviews, 51:158199.Google Scholar
Häder, D-P, Ed. 1992. Image analysis in biology. CRC Press.Google Scholar
Marchevsky, AM. and Bartels, PH. 1994. Image analysis: a primer for pathologists/editors, Raven Press.Google Scholar
Mariuzzi, GM. and Montironi, R. 1986. Quantitative microscopy and index formulation in continuous pathologic lesions. Appl. pathol. 4; 43-7.Google ScholarPubMed
Russ, JC. Computer-assisted microscopy: the measurement and analysis of images, Plenum Press. 1990.Google Scholar
Russ, JC. 1992. The image processing handbook, CRC Press.Google Scholar
Weibel 1973. In Principles and Techniques of EM. v 3, Ch. 6. Ed Hayat, Van Nostrand Reinhold.Google Scholar