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A Simple Technique For The Removal Of Plant Cell Protoplasm To Facilitate Scanning : Electron Microscopy Of Fungal Haustoria And Plant Cell Wall Features

Published online by Cambridge University Press:  14 March 2018

B. A. Richardson*
Affiliation:
University of Georgia
C. W. Mims
Affiliation:
University of Georgia

Extract

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Several years ago Honegger (1985) described a simple technique for removing plant cell protoplasm in order to reveal details of interfaces between plant cells and fungal structures. This technique involves the use of Ariel a commercially available washing powder (Proctor and Gamble) containing a Bacillus substilis derived protease. We since have used this technique with excellent results to examine not only the morphology of fungal haustoria inside leaf cells of various host plants but also features of the inner surface of the host cell wall with scanning electron microscopy (SEM). Here we describe the procedure we have used to prepare samples for study and provide examples of the types of images we have obtained from our samples.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2001