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A Tip on Purifying Glutaraldehyde

Published online by Cambridge University Press:  14 March 2018

Lawrence R Melsen*
Affiliation:
Emory University School Of Medicine

Extract

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Being an old timer, I have been around since before the advent of vials of glutaraldehyde. In the 1970s, the Merck index described a method for purifying glutaraldehyde using activated charcoal.

Basically, glutaraldehyde begins to polymerize when stored at room temperature. Since best fixation is achieved with the monomeric form, this purification protocol was designed to remove the more complex forms. Storage under nitrogen in a sealed vial slows the polymerization, and lower concentrations degrade more slowly. Storing at 4°C is advised.

Type
Microscopy 101
Copyright
Copyright © Microscopy Society of America 2000