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Polypropylene films modified by grafting-from of ethylene glycol dimethacrylate/glycidyl methacrylate using γ-rays and antimicrobial biofunctionalization by Schiff bases

Published online by Cambridge University Press:  29 January 2018

G.G. Flores-Rojas ,
F. López-Saucedo ,
J.E. López-Barriguete ,
T. Isoshima ,
M. Luna-Straffon  and
E. Bucio
G.G. Flores-Rojas*
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
F. López-Saucedo
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
J.E. López-Barriguete
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
T. Isoshima
Affiliation:
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
M. Luna-Straffon
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
E. Bucio*
Affiliation:
Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México CDMX 04510, México
*
Address all correspondence to G.G. Flores-Rojas and E. Bucio at ggabofo@hotmail.com, ebucio@nucleares.unam.mx
Address all correspondence to G.G. Flores-Rojas and E. Bucio at ggabofo@hotmail.com, ebucio@nucleares.unam.mx
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Abstract

The goal of this work was to provide antimicrobial activity to polypropylene by covalent immobilization of lysozyme. The first step was the grafting of ethylene glycol dimethacrylate and glycidyl methacrylate through “grafting-from” method by means of γ-rays. Then those chemical groups were activated to allow the immobilization of lysozyme by Schiff bases. The activity of lysozyme showed an improvement by the remaining double bonds from the grafting. Finally, the presence of lysozyme was confirmed by the hydrolysis of Micrococcus lysodeikticus at different temperatures, pH values, and cycles. The new materials were characterized by infrared spectroscopy, thermal analysis, contact angle, and by the surface morphology.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 1 , March 2018 , pp. 168 - 177
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Copyright
Copyright © Materials Research Society 2018 

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