Hostname: page-component-77c89778f8-n9wrp Total loading time: 0 Render date: 2024-07-19T05:29:37.788Z Has data issue: false hasContentIssue false
  • English
  • Français

“Green” electrospinning of a collagen/hydroxyapatite composite nanofibrous scaffold

Published online by Cambridge University Press:  26 September 2016

David A. Castilla-Casadiego ,
Michael Maldonado ,
Paul Sundaram  and
Jorge Almodovar
David A. Castilla-Casadiego
Affiliation:
Department of Chemical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico 00681-9000, USA
Michael Maldonado
Affiliation:
Department of Mechanical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico 00681-9000, USA
Paul Sundaram
Affiliation:
Department of Mechanical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico 00681-9000, USA
Jorge Almodovar*
Affiliation:
Department of Chemical Engineering, University of Puerto Rico Mayaguez, Call Box 9000, Mayaguez, Puerto Rico 00681-9000, USA
*
Address all correspondence to Jorge Almodovar at jorge.almodovar1@upr.edu
Get access

Abstract

In this work, a composite nanofibrous scaffold of collagen/hydroxyapatite was prepared by electrospinning using a mild solvent. Hydroxyapatite particles dispersed into a collagen/acetic acid/water solution was electrospun to yield composite nanofibers. Scanning electron microscopy reveals nanofibers with an average diameter of 342 ± 67 nm, and a rough surface caused by the hydroxyapatite particles. Both X-ray and infrared spectroscopy confirmed the presence of the hydroxyapatite particles embedded in the collagen fibers. The inclusion of hydroxyapatite particles does not alter the native collagen structure. Lastly, these composite nanofibers support pre-osteoblast adhesion. These results show how “green” electrospinning could be used to generate nanocomposite scaffolds with potential biomedical applications.

Type
Research Letters
Information
MRS Communications , Volume 6 , Issue 4 , December 2016 , pp. 402 - 407
Check for updates
Copyright
Copyright © Materials Research Society 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Agarwal, S., Wendorff, J.H., and Greiner, A.: Use of electrospinning technique for biomedical applications. Polymer (Guildf) 49, 5603–5621 (2008).Google Scholar
2. Walters, B.D. and Stegemann, J.P.: Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales. Acta Biomater. 10, 1488–1501 (2014).Google Scholar
3. Almodovar, J., Castilla Casadiego, D.A., and Ramos Avilez, H.V.: Polysaccharide-based biomaterials for cell-material interface. In Cell and Material Interface: Advances in Tissue Engineering, Biosensor, Implant, and Imaging Technologies, edited by Vrana, N.E. (CRC Press, 5, Florida, 2015), pp. 230–244.Google Scholar
4. Schiffman, J.D. and Schauer, C.L.: A review: electrospinning of biopolymer nanofibers and their applications. Polym. Rev. 48, 317–352 (2008).Google Scholar
5. Huang, G.P., Shanmugasundaram, S., Masih, P., Pandya, D., Amara, S., Collins, G., and Arinzeh, T.L.: An investigation of common crosslinking agents on the stability of electrospun collagen scaffolds. J. Biomed. Mater. Res. A 103, 762–771 (2015).Google Scholar
6. Rho, K.S., Jeong, L., Lee, G., Seo, B.-M., Park, Y.J., Hong, S.-D., Roh, S., Cho, J.J., Park, W.H., and Min, B.-M.: Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials 27, 1452–1461 (2006).Google Scholar
7. Zeugolis, D.I., Khew, S.T., Yew, E.S., Ekaputra, A.K., Tong, Y.W., Yung, L.-Y.L., Hutmacher, D.W., Sheppard, C., and Raghunath, M.: Electro-spinning of pure collagen nano-fibres – just an expensive way to make gelatin? Biomaterials 29, 2293–2305 (2008).Google Scholar
8. Liverani, L. and Boccaccini, A.: Versatile production of poly(Epsilon-Caprolactone) fibers by Electrospinning using benign solvents. Nanomaterials 6, 75 (2016).Google Scholar
9. Agarwal, S. and Greiner, A.: On the way to clean and safe electrospinning-green electrospinning: emulsion and suspension electrospinning. Polym. Adv. Technol. 22, 372–378 (2011).CrossRefGoogle Scholar
10. Kazanci, M.: Solvent and temperature effects on folding of electrospun collagen nanofibers. Mater. Lett. 130, 223–226 (2014).Google Scholar
11. Lin, J., Li, C., Zhao, Y., Hu, J., and Zhang, L.-M.: Co-electrospun nanofibrous membranes of collagen and Zein for wound healing. ACS Appl. Mater. Interfaces 4, 1050–1057 (2012).Google Scholar
12. Elamparithi, A., Punnoose, A.M., and Kuruvilla, S.: Electrospun type 1 collagen matrices preserving native ultrastructure using benign binary solvent for cardiac tissue engineering. Artif. Cells, Nanomed., Biotechnol. 44, 1–8 (2015).Google Scholar
13. Fiorani, A., Gualandi, C., Panseri, S., Montesi, M., Marcacci, M., Focarete, M.L., and Bigi, A.: Comparative performance of collagen nanofibers electrospun from different solvents and stabilized by different crosslinkers. J. Mater. Sci. Mater. Med. 25, 2313–2321 (2014).Google Scholar
14. Liu, T., Teng, W.K., Chan, B.P. and Chew, S.Y.: Photochemical crosslinked electrospun collagen nanofibers: synthesis, characterization and neural stem cell interactions. J. Biomed. Mater. Res. A 95 A, 276–282 (2010).Google Scholar
15. Castilla Casadiego, D., Ramos Avilez, H.V., Herrera-Posada, S., Calcagno, B., Loyo, L., Shipmon, J., Acevedo, A., Quintana, A., and Almodovar, J.: Engineering of a stable collagen nanofibrous scaffold with tunable fiber diameter, alignment, and mechanical properties. Macromol. Mater. Eng. 301, 1064–1075 (2016).Google Scholar
16. Young, M.F.: Bone matrix proteins: their function, regulation, and relationship to osteoporosis. Osteoporos. Int. 14(Suppl 3), S35–S42 (2003).Google Scholar
17. Teng, S.-H., Lee, E.-J., Wang, P., and Kim, H.-E.: Collagen/hydroxyapatite composite nanofibers by electrospinning. Mater. Lett. 62, 3055–3058 (2008).Google Scholar
18. Venugopal, J., Low, S., Choon, A.T., Sampath Kumar, T.S., and Ramakrishna, S.: Mineralization of osteoblasts with electrospun collagen/hydroxyapatite nanofibers. J. Mater. Sci. Mater. Med. 19, 2039–2046 (2008).Google Scholar
19. Ji, J., Bar-On, B., and Wagner, H.D.: Mechanics of electrospun collagen and hydroxyapatite/collagen nanofibers. J. Mech. Behav. Biomed. Mater. 13, 185–193 (2012).Google Scholar
20. Zhang, Y., Reddy, V.J., Wong, S.Y., Li, X., Su, B., Ramakrishna, S., and Lim, C.T.: Enhanced biomineralization in osteoblasts on a novel electrospun biocomposite nanofibrous substrate of hydroxyapatite/collagen/chitosan. Tissue Eng. A 16, 1949–1960 (2010).Google Scholar
21. Asran, A.S., Henning, S., and Michler, G.H.: Polyvinyl alcohol–collagen–hydroxyapatite biocomposite Nanofibrous scaffold: mimicking the key features of natural bone at the nanoscale level. Polymer (Guildf) 51, 868–876 (2010).Google Scholar
22. Song, W., Markel, D.C., Wang, S., Shi, T., Mao, G., and Ren, W.: Electrospun polyvinyl alcohol-collagen-hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells. Nanotechnology 23, 115–101 (2012).Google Scholar
23. Zhou, Y., Yao, H., Wang, J., Wang, D., Liu, Q., and Li, Z.: Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering. Int. J. Nanomed. 10, 3203–3215 (2015).Google Scholar
24. Towler, D.A. and Arnaud, R.S.: Use of cultured osteoblastic cells to identify and characterize transcriptional regulatory complexes. In Principles of Bone Biology, 2nd ed.; Bilezikian, J.P., Raisz, L.G. and Rodan, G.A., eds; Academic Press: London, England, 2002; pp. 1503–1527.Google Scholar
25. Camacho, N.P., West, P., Torzilli, P.A., and Mendelsohn, R.: FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. Biopolym.—Biospectrosc. Sect. 62, 1–8 (2001).Google Scholar
26. Trommer, R.M., dos Santos, L.A., and Bergmann, C.P.: Alternative technique to obtain hydroxyapatite coatings. Cerâmica 53, 153–158 (2007).Google Scholar
27. Apella, M., Venegas, S., and Rodenas, L.: Synthetic hydroxyapatite as a surface model of dental enamel and dentine. J. Argentine Chem. Soc. 97, 109–118 (2008).Google Scholar
28. Varma, H.K., Yokogawa, Y., Espinosa, F.F., Kawamoto, Y., Nishizawa, K., Nagata, F., and Kameyama, T.: Porous calcium phosphate coating over phosphorylated chitosan film by a biomimetic method. Biomaterials 20, 879–884 (1999).Google Scholar
29. Manjubala, I., Ponomarev, I., Wilke, I., and Jandt, K.D.: Growth of osteoblast-like cells on biomimetic apatite-coated chitosan scaffolds. J. Biomed. Mater. Res. B, Appl. Biomater. 84, 7–16 (2008).Google Scholar
30. Shah, N.J., Hong, J., Hyder, M.N., and Hammond, P.T.: Osteophilic multilayer coatings for accelerated bone tissue growth. Adv. Mater. 24, 1445–50 (2012).Google Scholar