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Cell Biology, Biochemistry and Genomics of Coccolithophore Biomineralization.

Published online by Cambridge University Press:  01 February 2011

Elma L. González  and
Betsy A. Read
Elma L. González
Affiliation:
Dept. Ecology and Evolutionary Biol., Univ. California, Los Angeles, 90095-1606; U.S.A.
Betsy A. Read
Affiliation:
Dept. of Biology, California State University, San Marcos, 91093-0016, U.S.A.
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Abstract

The coccoliths that form the outer cell covering of the unicellular coccolithophores are assembled in a coccolith vesicle. The coccolith vesicle maintains the conditions and protein carriers sufficient to import the raw materials (Ca2+ and CO32-) for calcite formation and, furthermore, to initiate and sustain mineralization. We have isolated the coccolith vesicle membrane on which a proton-pumping, vacuolar ATPase has been localized. The V-ATPase has been characterized and its membrane-spanning subunit c has been cloned and sequenced. We used the highly conserved sequence for designing and constructing probes and primers that allowed us to analyze expression patterns to show a direct relationship between expression of the gene and calcification. Our collaborative work has now turned to a genomics approach that will lead us to additional calcification-specific genes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K4.4
Copyright
Copyright © Materials Research Society 2005

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