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Detection of Cell Surface Protein with Surface Enhanced Raman Spectroscopy

Published online by Cambridge University Press:  01 February 2011

Li-Lin Tay ,
Qingyan Hu ,
Matthew Noestheden  and
John Pezacki
Li-Lin Tay
Affiliation:
lilin.tay@nrc-cnrc.gc.ca, National Research Council, Institute for Microstructural Sciences, Building M-50; Room 190P, 1200 Montreal Road, Ottawa, Ottawa, Ontario, K1A 0R6, Canada
Qingyan Hu
Affiliation:
qingyan.hu@nrc-cnrc.gc.ca, National Research Council, Ottawa, K1A 0R6, Canada
Matthew Noestheden
Affiliation:
matthew.noestheden@nrc-cnrc.gc.ca, National Research Council, Ottawa, K1A 0R6, Canada
John Pezacki
Affiliation:
john.pezacki@nrc-cnrc.gc.ca, National Research Council, Ottawa, K1A 0R6, Canada
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Abstract

In this study, HeLa cells were transfected to express a recombinant transmembrane domain of the platelet-derived growth factor receptor. Silver nanoparticle (Ag-NP) functionalized with terminal hydrazide was employed to specifically target the ketone-conjugated cell surface proteins. In addition, a nitrile functional group was incorporated onto the Ag nanoprobe to provide unique vibrational signature (∼ 2230 cm−1) in a window which is free from other cellular components. The designed Ag nanoprobe functions as both recognition and transduction elements in the detection of the expressed cell surface protein. Scanning electron microscopy and surface enhanced Raman spectroscopy (SERS) imaging of the nanoparticle (NP) labeled HeLa cells revealed that all the observed intense SERS signals originated from aggregated NPs. Although isolated Ag-NPs is capable of SERS enhancement, its enhancement factor is beyond the detestability of such imaging experiments. The results have implication in the future design of SERS nanosensors for in vivo detection application.

Keywords

Raman spectroscopybiologicalnanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 952: Symposium F – Integrated Nanosensors , 2006 , 0952-F06-04
Copyright
Copyright © Materials Research Society 2007

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References

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