Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-09T05:03:09.497Z Has data issue: false hasContentIssue false
  • English
  • Français

Distyrylbenzene oligoelectrolyte (DSBN+) for human cervical carcinoma cells imaging

Published online by Cambridge University Press:  09 October 2013

Roza Pawlowska ,
Paulina Gwozdzinska ,
Logan Garner  and
Arkadiusz Chworos
Roza Pawlowska
Affiliation:
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363 Lodz, Poland
Paulina Gwozdzinska
Affiliation:
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363 Lodz, Poland
Logan Garner
Affiliation:
Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, United States
Arkadiusz Chworos*
Affiliation:
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363 Lodz, Poland
*
*Email: achworos@cbmm.lodz.pl
Get access

Abstract

In the presented study, a new application for distyrylbenzene oligoelectrolyte, named DSBN+, as a marker for bioimaging is presented. DSBN+ is a water-soluble, conjugated oligoelectrolyte (COE) with novel photophysical and solvatochromatic properties. Previous studies have shown that this compound spontaneously inserts into bilayer membranes in both synthetic and microbial living systems and can facilitate visualization of cell membranes through fluorescence imaging. In the presented research, we seek to further study and exploit the multifunctional nature of DSBN+ in terms of membrane interactions and photophysical properties for visualization of membranous structures of more complex mammalian cells, namely a human cervical carcinoma (HeLa) cell line. Obtained results confirm the possibility of applying DSBN+ as a fluorescent dye for bioimaging of membranes in human cell cultures systems, both in live-cell imaging and in the studies required formaldehyde fixation. Due to the defined structure of this conjugated oligoelectrolyte we suspect that it will display organelle membrane selectivity, but this has to be further investigated.

Keywords

cellular (material type)optical propertiesbiological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium QQ – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 207 - 211
Copyright
Copyright © Materials Research Society 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albota, M, Beljonne, D, Brédas, JL, Ehrlich, JE, Fu, JY, Heikal, AA, Hess, SE, Kogej, T, Levin, MD, Marder, SR, McCord-Maughon, D, Perry, JW, Röckel, H, Rumi, M, Subramaniam, G, Webb, WW, Wu, XL, Xu, C, Science, 281, 1653–1656 (1998)CrossRefGoogle Scholar
Woo, HY, Liu, B, Kohler, B, Korystov, D, Mikhailovsky, A, Bazan, GC, J. Am. Chem. Soc. 127, 14721–14729 (2005)10.1021/ja052906gCrossRefGoogle Scholar
Woo, HY, Korystov, D, Mikhailovsky, A, Nguyen, TQ, Bazan, GC, J. Am. Chem. Soc. 127, 13794–13795 (2005)CrossRefGoogle Scholar
Garner, LE, Park, J, Dyar, SM, Chworos, A, Sumner, JJ, Bazan, GC, J. Am. Chem. Soc. 132, 10042–10052 (2010)CrossRefGoogle Scholar
Ortony, JH, Chatterjee, T, Garner, LE, Chworos, A, Mikhailovsky, A, Kramer, EJ and Bazan, GC, J. Am. Chem. Soc. 133, 8380–8387 (2011)CrossRefGoogle Scholar
Schnell, U, Dijk, F, Sjollema, , Giepmans, BNG, Nat Methods. 9, 152–158 (2012)CrossRefGoogle Scholar
Massoud, TF, Gambhir, SS, Genes Dev. 17, 545–580 (2003)CrossRefGoogle Scholar